The effects of hydroalcoholic extract of Vaccinium arctostaphylos L. on sperm parameters, oxidative injury and apoptotic changes in oxymetholone-induced testicular toxicity in mouse.
Animals
Apoptosis
/ drug effects
Cell Survival
/ drug effects
Disease Models, Animal
Ethanol
/ chemistry
Fruit
/ chemistry
Humans
Infertility, Male
/ chemically induced
Male
Mice
Oxidative Stress
/ drug effects
Oxymetholone
/ toxicity
Plant Extracts
/ isolation & purification
Protective Agents
/ isolation & purification
Sperm Count
Sperm Motility
/ drug effects
Testis
/ cytology
Vaccinium
/ chemistry
Water
/ chemistry
Vaccinium arctostaphylos
oxymetholone
sperm parameter
stereology
testis
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
29
10
2019
revised:
07
12
2019
accepted:
16
12
2019
pubmed:
6
2
2020
medline:
25
11
2020
entrez:
4
2
2020
Statut:
ppublish
Résumé
Anabolic androgenic steroids (AAS) such as oxymetholone (OM) used for athletic enhancement, but increased free radicals damage and changes in hormonal levels, lead to serious and irreversible organ damage. Vaccinium arctostaphylos(V. arctostaphylos( has been demonstrated to have antioxidant and antiinflammatory effects. The aim of present study was to investigate V. arctostaphylos effect on OM-induced oxidative injury in mouse testis and sperm parameters. In this experimental study, 30 BALB/c mice were divided into five groups, including healthy, positive control(5 mg/kg OM) and three treatment groups (100, 200 and 400 mg/kg of V. arctostaphylos extract + 5 mg/kg OM). At the end of the study, serum luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone levels were measured. Testis stereological and sperm parameters were calculated. Antioxidant status was measured using nitric oxide (NO) and FRAP assay, and malondialdehyde (MDA) levels. Furthermore, the expression of p53, caspase-3, Bax and Bcl-2 was measured. V. arctostaphylos decreased the serum level of testosterone, increased the LH and FSH, and improved the stereological and sperm parameters and down-regulated the p53, caspase-3 and Bax and up-regulated Bcl-2 genes. Furthermore, this dose decreased serum levels of NO and increased testis FRAP and MDA levels in treated groups compared with OM group. V. arctostaphylos extract has protective effects against testicular toxicity caused by OM.
Substances chimiques
Plant Extracts
0
Protective Agents
0
Water
059QF0KO0R
Ethanol
3K9958V90M
Oxymetholone
L76T0ZCA8K
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13522Subventions
Organisme : Kermanshah University of Medical Sciences
ID : 3009071
Informations de copyright
© 2020 Blackwell Verlag GmbH.
Références
Akbari Bazm, M., Khazaei, M., Khazaei, F., & Naseri, L. (2019). Nasturtium Officinale L. hydroalcoholic extract improved oxymetholone-induced oxidative injury in mouse testis and sperm parameters. Andrologia, 51(7), e13294.
Akbari, M., Goodarzi, N., & Tavafi, M. (2017). Stereological assessment of normal Persian squirrels (Sciurus anomalus) kidney. Anatomical Science International, 92(2), 267-274. https://doi.org/10.1007/s12565-016-0332-3
Arabi, O. H., Kahil, M. A., & Ibrahim, N. A. (2014). The Effects of Aqueous Extract Hibiscus sabdariffa on Spermatogenesis and Sperm Parameters of Mice. International Journal of Current Research and Review, 6(10), 51.
Ayaz, F. A., Hayirlioglu-Ayaz, S., Gruz, J., Novak, O., & Strnad, M. (2005). Separation, characterization, and quantitation of phenolic acids in a little-known blueberry (Vaccinium arctostaphylos L.) fruit by HPLC-MS. Journal of Agricultural and Food Chemistry, 53(21), 8116-8122.
Bahrke, M. S., & Yesalis, C. E. (2004). Abuse of anabolic androgenic steroids and related substances in sport and exercise. Current Opinion in Pharmacology, 4(6), 614-620. https://doi.org/10.1016/j.coph.2004.05.006
Bazm, M. A., Khazaei, M., Ghanbari, E., & Naseri, L. (2018). Protective effect of Vaccinium arctostaphylos L. fruit extract on gentamicin-induced nephrotoxicity in rats. Comparative Clinical Pathology, 27(5), 1327-1334.
Beato, M., Herrlich, P., & Schütz, G. (1995). Steroid hormone receptors: Many actors in search of a plot. Cell, 83(6), 851-857. https://doi.org/10.1016/0092-8674(95)90201-5
Bhushan, S., Aslani, F., Zhang, Z., Sebastian, T., Elsaesser, H. P., & Klug, J. (2016). Isolation of sertoli cells and peritubular cells from rat testes. Journal of Visualized Experiments, 108, e53389-e53389.
Christou, M. A., Christou, P. A., Markozannes, G., Tsatsoulis, A., Mastorakos, G., & Tigas, S. (2017). Effects of anabolic androgenic steroids on the reproductive system of athletes and recreational users: A systematic review and meta-analysis. Sports Medicine, 47(9), 1869-1883. https://doi.org/10.1007/s40279-017-0709-z
Clark, A. S., Blasberg, M. E., & Brandling-Bennett, E. M. (1998). Stanozolol, oxymetholone, and testosterone cypionate effects on the rat estrous cycle. Physiology & Behavior, 63(2), 287-295. https://doi.org/10.1016/S0031-9384(97)00443-5
Cooper, T. G., Noonan, E., von Eckardstein, S., Auger, J., Baker, H. W. G., Behre, H. M., … Vogelsong, K. M. (2010). World Health Organization reference values for human semen characteristics. Human Reproduction Update, 16(3), 231-245. https://doi.org/10.1093/humupd/dmp048
De Souza, G. L., & Hallak, J. (2011). Anabolic steroids and male infertility: A comprehensive review. BJU International, 108(11), 1860-1865. https://doi.org/10.1111/j.1464-410X.2011.10131.x
Duan, P., Hu, C., Butler, H. J., Quan, C., Chen, W., Huang, W., … Martin, F. L. (2017). 4-Nonylphenol induces disruption of spermatogenesis associated with oxidative stress-related apoptosis by targeting p53-Bcl-2/Bax-Fas/FasL signaling. Environmental Toxicology, 32(3), 739-753.
Eleawa, S. M., Alkhateeb, M. A., Alhashem, F. H., Bin-jaliah, I., Sakr, H. F., Elrefaey, H. M., … Khalil, M. A. (2014). Resveratrol reverses cadmium chloride-induced testicular damage and subfertility by downregulating p53 and Bax and upregulating gonadotropins and Bcl-2 gene expression. Journal of Reproduction and Development, 60(2), 115-127. https://doi.org/10.1262/jrd.2013-097
El-hanbuli, H. M., Abo-sief, A. F., & Mostafa, T. (2017). Protective effect of silymarin on the testes of rats treated with anabolic androgenic steroid: A biochemical, histological, histochemical and immunohistochemical study. Journal of Histology & Histopathology, 4(1), 10.
Ghanbari, E., Nejati, V., & Khazaei, M. (2016). Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats. International Journal of Reproductive BioMedicine, 14(8), 519-526. https://doi.org/10.29252/ijrm.14.8.519
Ghanbari, M., Mortazavi, S. B., Khavanin, A., & Khazaei, M. (2012). Simultaneous effects of exposure to microwaves and noise on male rat's perm parameters and total antioxidant capacity. Health Scope, 1(4), 180-186.
Han, Y. T., Chen, X. H., Xie, J., Zhan, S. M., Wang, C. B., & Wang, L. X. (2011). Purple sweet potato pigments scavenge ROS, reduce p53 and modulate Bcl-2/Bax to inhibit irradiation-induced apoptosis in murine thymocytes. Cellular Physiology and Biochemistry, 28(5), 865-872. https://doi.org/10.1159/000335801
Hartgens, F., & Kuipers, H. (2004). Effects of androgenic-anabolic steroids in athletes. Sports Medicine, 34(8), 513-554. https://doi.org/10.2165/00007256-200434080-00003
Jang, H., Kim, S. J., Yuk, S. M., Han, D. S., Ha, U.-S., Hong, S. H., … Kim, S. W. (2012). Effects of anthocyanin extracted from black soybean seed coat on spermatogenesis in a rat varicocele-induced model. Reproduction, Fertility and Development, 24(5), 649-655. https://doi.org/10.1071/RD11174
Janjic, M. M., Stojkov, N. J., Andric, S. A., & Kostic, T. S. (2012). Anabolic-androgenic steroids induce apoptosis and NOS2 (nitric-oxide synthase 2) in adult rat Leydig cells following in vivo exposure. Reproductive Toxicology, 34(4), 686-693. https://doi.org/10.1016/j.reprotox.2012.10.003
Katsube, N., Iwashita, K., Tsushida, T., Yamaki, K., & Kobori, M. (2003). Induction of apoptosis in cancer cells by bilberry (Vaccinium myrtillus) and the anthocyanins. Journal of Agricultural and Food Chemistry, 51(1), 68-75.
Khazaei, M. R., Rashidi, Z., Chobsaz, F., & Khazaei, M. (2016). Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine. Iranian Journal of Basic Medical Sciences, 19(9), 940.
Kim, S. K., Yoon, Y. D., Park, Y. S., Seo, J. T., & Kim, J. H. (2007). Involvement of the Fas-Fas ligand system and active caspase-3 in abnormal apoptosis in human testes with maturation arrest and Sertoli cell-only syndrome. Fertility and Sterility, 87(3), 547-553. https://doi.org/10.1016/j.fertnstert.2006.07.1524
Latti, A. K., Kainulainen, P. S., Hayirlioglu-Ayaz, S., Ayaz, F. A., & Riihinen, K. R. (2009). Characterization of anthocyanins in Caucasian blueberries (Vaccinium arctostaphylos L.) native to Turkey. Journal of Agricultural and Food Chemistry, 57(12), 5244-5249.
Li, G. Y., Xie, P., Li, H. Y., Hao, L., Xiong, Q., & Qiu, T. (2011). Involment of p53, Bax, and Bcl-2 pathway in microcystins-induced apoptosis in rat testis. Environmental Toxicology, 26(2), 111-117. https://doi.org/10.1002/tox.20532
Li, P. F., Dietz, R., & von Harsdorf, R. (1999). p53 regulates mitochondrial membrane potential through reactive oxygen species and induces cytochrome c-independent apoptosis blocked by Bcl-2. The EMBO Journal, 18(21), 6027-6036. https://doi.org/10.1093/emboj/18.21.6027
Lopez-Martinez, L. X., Oliart-Ros, R. M., Valerio-Alfaro, G., Lee, C. H., Parkin, K. L., & Garcia, H. S. (2009). Antioxidant activity, phenolic compounds and anthocyanins content of eighteen strains of Mexican maize. LWT-Food Science and Technology, 42(6), 1187-1192. https://doi.org/10.1016/j.lwt.2008.10.010
Mahboubi, M., Kazempour, N., & Taghizadeh, M. (2013). In vitro antimicrobial and antioxidant activity of Vaccinium arctostaphylos L. extracts. Journal of Biologically Active Products from. Nature, 3(4), 241-247.
Matzuk, M. M., & Lamb, D. J. (2008). The biology of infertility: Research advances and clinical challenges. Nature Medicine, 14(11), 1197. https://doi.org/10.1038/nm.f.1895
Mehrabinasab, E., Khazaei, M., & Khazaei, S. (2010). The effect of pentylenetetrazol kindling induced epilepsy on hypogonad hormones and sperm parameters of rats.
Najafi, G., Nejati, V., Shalizar Jalali, A., & Zahmatkesh, E. (2014). Protective role of royal jelly in oxymetholone-induced oxidative injury in mouse testis. Iranian Journal of Toxicology, 8(25), 1073-1080.
Naseri, L., Khazaei, M., Ghanbari, E., & Bazm, M. A. (2019). Rumex alveollatus hydroalcoholic extract protects CCL4-induced hepatotoxicity in mice. Comparative Clinical Pathology, 28(2), 557-565. https://doi.org/10.1007/s00580-018-2846-7
Nickavar, B., & Amin, G. (2004). Anthocyanins from Vaccinium arctostaphylos berries. Pharmaceutical Biology, 42(4-5), 289-291.
Nickavar, B., & Amin, G. (2011). Enzyme assay guided isolation of an α-amylase inhibitor flavonoid from Vaccinium arctostaphylos leaves. Iranian Journal of Pharmaceutical Research: IJPR, 10(4), 849.
Omotuyi, I. O., Ologundudu, A., Onwubiko, V. O., Wogu, M. D., & Obi, F. O. (2010). Hibiscus sabdariffa Linn. anthocyanins alter circulating reproductive hormones in rabbits (Oryctolagus cuniculus). Journal of Diabetes and Endocrinology, 1(3), 36-45.
Pavlatos, A. M., Fultz, O., Monberg, M. J., & Vootkur, A. (2001). Review of oxymetholone: A 17α-alkylated anabolic-androgenic steroid. Clinical Therapeutics, 23(6), 789-801. https://doi.org/10.1016/S0149-2918(01)80070-9
Ravan, A. P., Bahmani, M., Basir, H. R. G., Salehi, I., & Oshaghi, E. A. (2017). Hepatoprotective effects of Vaccinium arctostaphylos against CCl4-induced acute liver injury in rats. Journal of Basic and Clinical Physiology and Pharmacology, 28(5), 463-471. https://doi.org/10.1515/jbcpp-2016-0181
Rigi Manesh, M., Arab, M. R., Sargolzaei Aval, F., Mashhadi, M. A., Sargolzaei, N., Mir, M., & Shahraki Salar, M. (2017). Cisplatin induce changes of cell surface glycoconjugates in germinal epithelium of seminiferous tubules. Anatomical Sciences Journal, 14(1), 31-36.
Rojas-García, P. P., Recabarren, M. P., Sarabia, L., Schön, J., Gabler, C., Einspanier, R., … Recabarren, S. E. (2010). Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams. American Journal of Physiology-Endocrinology and Metabolism, 299(6), E998-E1005. https://doi.org/10.1152/ajpendo.00032.2010
Sanocka, D., & Kurpisz, M. (2004). Reactive oxygen species and sperm cells. Reproductive Biology and Endocrinology, 2(1), 12.
Sedaghathoor, S. H. A. H. R. A. M., Kashi, A. K., Talaei, A. R., & Khalighi, A. (2006). Essential oils of Qare-Qat (Vaccinium arctostaphylos) shoots and chemical composition of berries. International Journal of Agriculture and Biology, 8, 45-46.
Singh, R., Singh, S., Jeyabalan, G., & Ali, A. (2012). An overview on traditional medicinal plants as aphrodisiac agent. Journal of Pharmacognosy and Phytochemistry, 1(4), 43-56.
Torres-Calleja, J., Gonzalez-Unzaga, M., DeCelis-Carrillo, R., Calzada-Sanchez, L., & Pedron, N. (2001). Effect of androgenic anabolic steroids on sperm quality and serum hormone levels in adult male bodybuilders. Life Sciences, 68(15), 1769-1774. https://doi.org/10.1016/S0024-3205(01)00972-9
Turillazzi, E., Perilli, G., Di Paolo, M., Neri, M., Riezzo, I., & Fineschi, V. (2011). Side effects of AAS abuse: An overview. Mini Reviews in Medicinal Chemistry, 11(5), 374-389.
Van Amsterdam, J., Opperhuizen, A., & Hartgens, F. (2010). Adverse health effects of anabolic-androgenic steroids. Regulatory Toxicology and Pharmacology, 57(1), 117-123. https://doi.org/10.1016/j.yrtph.2010.02.001
Woo, A. L., James, P. F., & Lingrel, J. B. (2000). Sperm motility is dependent on a unique isoform of the Na. K-ATPase. Journal of Biological Chemistry, 275(27), 20693-20699. https://doi.org/10.1074/jbc.M002323200
Zhang, Z. H., Zhou, X. C., Wei, P., Hu, Z. Y., & Liu, Y. X. (2003). Expression of Bcl-2 and Bax in rhesus monkey testis during germ cell apoptosis induced by testosterone undecanoate. Archives of Andrology, 49(6), 439-447. https://doi.org/10.1080/01485010390219836