Characterization of three-dimensional rat central nervous system culture maturation, with applications to monitor cholinergic integrity.
CNS 3-dimensional culture
acetylcholine esterase
mature-like tissue
neuropathology model
reaggregate
synapse integrity
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
25
05
2019
revised:
19
01
2020
accepted:
23
01
2020
pubmed:
6
2
2020
medline:
5
8
2021
entrez:
4
2
2020
Statut:
ppublish
Résumé
Studying age-related neuropathologies in vitro requires a three-dimensional (3D) culture system presenting mature phenotypes. In this study, we aimed to determine whether aged reaggregate cultures physiologically represent mature brain tissue. Results support that embryo-derived rat central nervous system (CNS) reaggregate cultures develop into mature-like tissues, comparable to in vivo maturation, including the following characteristics: (a) progressive reduction in cell proliferation (reduced anti-Ki-67 immunoreactivity), (b) progressive restriction of long neurite growth potential (as explant cultures), and (c) increased and sustained synaptic enzyme (acetylcholine esterase, AChE) activity. The acquisition of mature-like reaggregate cultures has allowed us to pursue the hypothesis that the physiological integrity of 3D CNS cultures may be monitored by synaptic enzyme activity. To assess this hypothesis, mature-like reaggregates were exposed to H
Substances chimiques
Glutamic Acid
3KX376GY7L
Acetylcholinesterase
EC 3.1.1.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2976Subventions
Organisme : Research Center on Aging
Pays : International
Informations de copyright
© 2020 American Institute of Chemical Engineers.
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