Controlled heating and alignment platform enhances versatility in colloidal probe fabrication.

A colloidal probe, comprising a colloidal particle attached to an atomic force microscope cantilever, is employed to measure interaction forces between the particle and a surface. It is possible to change or even destroy a particle while attaching it to a cantilever, thus limiting the types of systems to which the colloidal probe technique may be applied. Here, we present the Controlled Heating and Alignment Platform (CHAP) for fabricating colloidal probes without altering the original characteristics of the attached particle. The CHAP applies heat directly to the atomic force microscope chip to rapidly and precisely control the cantilever temperature. It minimizes particle heating and enables control over the viscosity of the thermoplastic adhesive to prevent it from contaminating the particle surface. 3D-printed components made the CHAP compatible with standard optical microscopes and streamlined the fabrication process, while increasing the platform's versatility. To demonstrate the utility of CHAP, we conducted a case study using a thermoplastic wax adhesive to fabricate colloidal probes bearing polystyrene and silica particles between 0.7 and 40 μm in diameter. We characterized the properties and interactions of the adhesive and particles, as well as the properties of the completed probes, to demonstrate the retention of particle features throughout fabrication. Pull-off tests with CHAP's probes measured adhesive force values in the expected ranges and demonstrated that particles were firmly attached to the cantilevers.