Emerging PCR-Based Techniques to Study HIV-1 Reservoir Persistence.
HIV-1
HIV-1 genome
HIV-1 reservoir
NGS
PCR
integration site
replication-competent
sequencing
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
28 01 2020
28 01 2020
Historique:
received:
20
12
2019
revised:
24
01
2020
accepted:
26
01
2020
entrez:
5
2
2020
pubmed:
6
2
2020
medline:
20
2
2021
Statut:
epublish
Résumé
While current antiretroviral therapies are able to halt HIV-1 progression, they are not curative, as an interruption of treatment usually leads to viral rebound. The persistence of this stable HIV-1 latent reservoir forms the major barrier in HIV-1 cure research. The need for a better understanding of the mechanisms behind reservoir persistence resulted in the development of several novel assays allowing to perform an extensive in-depth characterization. The objective of this review is to present an overview of the current state-of-the-art PCR-based technologies to study the replication-competent HIV-1 reservoir. Here, we outline the advantages, limitations, and clinical relevance of different approaches. Future HIV-1 eradication studies would benefit from information-rich, high-throughput assays as they provide a more efficient and standardized way of characterizing the persisting HIV-1 reservoir.
Identifiants
pubmed: 32012811
pii: v12020149
doi: 10.3390/v12020149
pmc: PMC7077278
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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