Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter.
Bayesian statistical analysis
curvature effect
high-throughput proteomics
protein corona
silica nanoparticles
Journal
Nanomaterials (Basel, Switzerland)
ISSN: 2079-4991
Titre abrégé: Nanomaterials (Basel)
Pays: Switzerland
ID NLM: 101610216
Informations de publication
Date de publication:
29 Jan 2020
29 Jan 2020
Historique:
received:
18
12
2019
revised:
17
01
2020
accepted:
22
01
2020
entrez:
5
2
2020
pubmed:
6
2
2020
medline:
6
2
2020
Statut:
epublish
Résumé
Biomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of different sizes interacting with soluble yeast protein extracts. Adsorption isotherms showed that the amount of adsorbed proteins varied greatly upon NP size with large NPs having more adsorbed proteins per surface unit. The protein corona composition was studied using a large-scale label-free proteomic approach, combined with statistical and regression analyses. Most of the proteins adsorbed on the NPs were the same, regardless of the size of the NPs. To go beyond, the protein physicochemical parameters relevant for the adsorption were studied: electrostatic interactions and disordered regions are the main driving forces for the adsorption on SiNPs but polypeptide sequence length seems to be an important factor as well. This article demonstrates that curvature effects exhibited using model proteins are not determining factors for the corona composition on SiNPs, when dealing with complex biological media.
Identifiants
pubmed: 32013169
pii: nano10020240
doi: 10.3390/nano10020240
pmc: PMC7075126
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Commissariat à l'Énergie Atomique et aux Énergies Alternatives
ID : Programme de Toxicologie/NaToM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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