Discovery of several thousand highly diverse circular DNA viruses.
evolutionary biology
infectious disease
metagenomics
microbiology
microbiome
viral evolution
virus
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
04 02 2020
04 02 2020
Historique:
received:
19
09
2019
accepted:
06
01
2020
entrez:
5
2
2020
pubmed:
6
2
2020
medline:
10
4
2021
Statut:
epublish
Résumé
Although millions of distinct virus species likely exist, only approximately 9000 are catalogued in GenBank's RefSeq database. We selectively enriched for the genomes of circular DNA viruses in over 70 animal samples, ranging from nematodes to human tissue specimens. A bioinformatics pipeline, Cenote-Taker, was developed to automatically annotate over 2500 complete genomes in a GenBank-compliant format. The new genomes belong to dozens of established and emerging viral families. Some appear to be the result of previously undescribed recombination events between ssDNA and ssRNA viruses. In addition, hundreds of circular DNA elements that do not encode any discernable similarities to previously characterized sequences were identified. To characterize these 'dark matter' sequences, we used an artificial neural network to identify candidate viral capsid proteins, several of which formed virus-like particles when expressed in culture. These data further the understanding of viral sequence diversity and allow for high throughput documentation of the virosphere.
Identifiants
pubmed: 32014111
doi: 10.7554/eLife.51971
pii: 51971
pmc: PMC7000223
doi:
pii:
Substances chimiques
Capsid Proteins
0
DNA, Circular
0
DNA, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIA NIH HHS
ID : P30 AG066507
Pays : United States
Déclaration de conflit d'intérêts
MT, DP, NW, BS, AP, GS, YP, SK, PP, DM, PM, JW, BM, JB, SR, BR, JD, BT, OP, JT, SR, BB, MS, AV, AS, CB No competing interests declared
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