Comparative evaluation of fluorescence polarization assay and competitive ELISA for the diagnosis of bovine brucellosis vis-a-vis sero-monitoring.
Animals
Antibodies, Bacterial
/ blood
Bacterial Vaccines
/ immunology
Brucella abortus
/ immunology
Brucellosis, Bovine
/ diagnosis
Cattle
Enzyme-Linked Immunosorbent Assay
/ methods
Fluorescence Polarization Immunoassay
/ methods
Mass Screening
/ methods
Sensitivity and Specificity
Serologic Tests
/ veterinary
Vaccination
/ veterinary
FPA
RBPT
Seroprevalence
cELISA
iELISA
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
04
11
2019
revised:
30
01
2020
accepted:
31
01
2020
pubmed:
6
2
2020
medline:
20
4
2021
entrez:
5
2
2020
Statut:
ppublish
Résumé
Brucellosis is an important zoonosis that constitutes a serious public health hazard which is caused by a bacterium belonging to the genus Brucella. In the present study, two highly specific serological tests for brucellosis diagnosis, fluorescence polarization assay (FPA) and competitive ELISA (cELISA) were standardized in the laboratory, evaluated and compared with rose bengal plate test (RBPT), indirect ELISA (iELISA) and commercial cELISA kit. For test evaluation, 1386 serum samples [apparently healthy animals (n = 260), samples from Brucella infected farms (n = 701) and B. abortus S19 vaccinated animals (n = 425)] were analyzed to assess suitable diagnostic test in B. abortus S19 post vaccinated bovine population. In apparently healthy brucellosis free farms, RBPT, iELISA, in-house FPA and cELISA were found to be highly specific than commercial cELISA. Commercial cELISA kit was comparatively more sensitive than other serological tests in samples collected from infected farms. The FPA showed sensitivity nearly equal to RBPT and in-house cELISA showed greater sensitivity than RBPT in infected farms. In animals with persistent vaccinal antibodies, only in-house FPA and cELISA recorded higher specificity of 87.64 and 90.27%, respectively. The other tests, RBPT and iELISA displayed similar reactivity with vaccine antibodies to that of infection antibodies whereas commercial cELISA kit showed an intermediate specificity of 47.69%. With these findings, RBPT, iELISA and cELISA are suggested for screening infected herds, and in-house developed FPA and cELISA tests with a proven specificity can be used for confirmatory diagnosis of brucellosis in B. abortus S19 post vaccinated animal populations.
Identifiants
pubmed: 32014437
pii: S0167-7012(19)30981-9
doi: 10.1016/j.mimet.2020.105858
pii:
doi:
Substances chimiques
Antibodies, Bacterial
0
Bacterial Vaccines
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105858Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest We declare that authors of the manuscript have no conflicts of interest.