Purification of Recombinant Adeno-Associated Viruses (rAAVs) by Iodixanol Gradient Centrifugation.
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
03 02 2020
03 02 2020
Historique:
entrez:
5
2
2020
pubmed:
6
2
2020
medline:
5
6
2021
Statut:
epublish
Résumé
This is a simple method for rapid preparation of recombinant adeno-associated virus (rAAV) stocks, which can be used for in vivo gene delivery. The purity of these vectors is considerably lower than that obtained by either CsCl gradient centrifugation or by combination of iodixanol gradient ultracentrifugation followed by column chromatography.
Identifiants
pubmed: 32015002
pii: 2020/2/pdb.prot095612
doi: 10.1101/pdb.prot095612
doi:
Substances chimiques
Chlorides
0
Triiodobenzoic Acids
0
Cesium
1KSV9V4Y4I
cesium chloride
GNR9HML8BA
iodixanol
HW8W27HTXX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
095612Subventions
Organisme : NINDS NIH HHS
ID : R01 NS076991
Pays : United States
Informations de copyright
© 2020 Cold Spring Harbor Laboratory Press.