Resistance to change: AMR gene dynamics on a commercial pig farm with high antimicrobial usage.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 02 2020
03 02 2020
Historique:
received:
28
10
2019
accepted:
19
01
2020
entrez:
5
2
2020
pubmed:
6
2
2020
medline:
20
11
2020
Statut:
epublish
Résumé
Group antimicrobial administration is used to control disease in livestock, but we have little insight into how this impacts antimicrobial resistance (AMR) gene dynamics. Here, a longitudinal study was carried out during a single production cycle on a commercial pig unit with high historic and current antimicrobial usage. Quantitative PCR, 16S rRNA gene metabarcoding and shotgun metagenomic sequencing were used to track faecal AMR gene abundance and diversity and microbiome alpha diversity. Shotgun metagenomic sequencing identified 144 AMR genes in total, with higher AMR gene diversity present in young pigs compared to dry sows. Irrespective of in-feed antibiotic treatment or changes in microbiome diversity, mean AMR gene copy number was consistently high, with some AMR genes present at copy numbers comparable to the bacterial 16S rRNA gene. In conclusion, AMR gene prevalence and abundance were not influenced by antibiotic use, either during the production cycle or following whole-herd medication. The high levels of certain genes indicate they are widely disseminated throughout the microbial population, potentially aiding stability. Despite the high and relatively stable levels of resistance genes against the main antimicrobials used, these compounds continue to control production limiting diseases on this unit.
Identifiants
pubmed: 32015392
doi: 10.1038/s41598-020-58659-3
pii: 10.1038/s41598-020-58659-3
pmc: PMC6997390
doi:
Substances chimiques
Anti-Infective Agents
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1708Subventions
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/P007767/1
Pays : International
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/J004227/1
Pays : International
Organisme : RCUK | Natural Environment Research Council (NERC)
ID : NE/N020162/1
Pays : International
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