Neuronal firing modulation by a membrane-targeted photoswitch.
Journal
Nature nanotechnology
ISSN: 1748-3395
Titre abrégé: Nat Nanotechnol
Pays: England
ID NLM: 101283273
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
04
09
2018
accepted:
18
12
2019
pubmed:
6
2
2020
medline:
18
11
2020
entrez:
5
2
2020
Statut:
ppublish
Résumé
Optical technologies allowing modulation of neuronal activity at high spatio-temporal resolution are becoming paramount in neuroscience. In this respect, azobenzene-based photoswitches are promising nanoscale tools for neuronal photostimulation. Here we engineered a light-sensitive azobenzene compound (Ziapin2) that stably partitions into the plasma membrane and causes its thinning through trans-dimerization in the dark, resulting in an increased membrane capacitance at steady state. We demonstrated that in neurons loaded with the compound, millisecond pulses of visible light induce a transient hyperpolarization followed by a delayed depolarization that triggers action potential firing. These effects are persistent and can be evoked in vivo up to 7 days, proving the potential of Ziapin2 for the modulation of membrane capacitance in the millisecond timescale, without directly affecting ion channels or local temperature.
Identifiants
pubmed: 32015505
doi: 10.1038/s41565-019-0632-6
pii: 10.1038/s41565-019-0632-6
doi:
Substances chimiques
Azo Compounds
0
azobenzene
F0U1H6UG5C
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
296-306Références
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