Investigations into Enhancing Yersinia pestis Cells Viability following Environmental Sampling for Forensic Analysis.
Yersinia pestis
BW detection
environmental sampling
forensic science
viability preservation
Journal
Journal of forensic sciences
ISSN: 1556-4029
Titre abrégé: J Forensic Sci
Pays: United States
ID NLM: 0375370
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
27
08
2019
revised:
07
01
2020
revised:
03
12
2019
accepted:
07
01
2020
pubmed:
6
2
2020
medline:
26
1
2021
entrez:
5
2
2020
Statut:
ppublish
Résumé
Following an intentional or accidental bio-warfare agent (BWA) release, environmental sample analysis is absolutely critical to determine the extent of contamination. When dealing with nonspore forming BWA (e.g., Yersinia pestis), retention of cell viability is central to such analyses. Even though significant advances have been achieved in DNA sequencing technologies, a positive identification of BWAs in environmental samples must be made through the ability of cells to form colony-forming units upon culturing. Inability to revive the cells between collection and analysis renders such studies inconclusive. Commercial kits designed to preserve the viability of pathogens contained within clinical samples are available, but many of them have not been examined for their ability to preserve samples containing suspected BWAs. The study was initiated to examine the applicability of commercial solutions aiding in retention of Y. pestis viability in samples stored under nonpermissive temperatures, that is, 40 and 37°C. While none of the tested solutions sustained cell viability at 40°C, the results show five out of 17 tested preservatives were capable of supporting viability of Y. pestis at 37°C.
Identifiants
pubmed: 32017101
doi: 10.1111/1556-4029.14293
doi:
Substances chimiques
Biological Warfare Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1315-1323Subventions
Organisme : Defense Threat Reduction Agency
ID : CBA #10239, 2016
Informations de copyright
© 2020 American Academy of Forensic Sciences. This article has been contributed to by US Government employees and their work is in the public domain in the USA.
Références
Edmonds JM. Efficient methods for large-area surface sampling of sites contaminated with pathogenic microorganisms and other hazardous agents: current state, needs, and perspectives. Appl Microbiol Biotechnol 2009;84(5):811-6.
Piepel GF, Amidan BG, Hu R. Laboratory studies on surface sampling of Bacillus anthracis contamination: summary, gaps and recommendations. J Appl Microbiol 2012;113(6):1287-304.
Charalambous BM, Batt SL, Peek AC, Mwerinde H, Sam N, Gillespie SH. Quantitative validation of media for transportation and storage of Streptococcus pneumoniae. J Clin Microbiol 2003;41(12):5551-6.
Drake C, Barenfanger J, Lawhorn J, Verhulst S. Comparison of Easy-Flow Copan Liquid Stuart's and Starplex Swab transport systems for recovery of fastidious aerobic bacteria. J Clin Microbiol 2005;43(3):1301-3.
Farhat SE, Thibault M, Devlin R. Efficacy of a swab transport system in maintaining viability of Neisseria gonorrhoeae and Streptococcus pneumoniae. J Clin Microbiol 2001;39(8):2958-60.
Freeman J, Wilcox MH. The effects of storage conditions on viability of Clostridium difficile vegetative cells and spores and toxin activity in human faeces. J Clin Pathol 2003;56(2):126-8.
Graver MA, Wade JJ. Survival of Neisseria gonorrhoeae isolates of different auxotypes in six commercial transport systems. J Clin Microbiol 2004;42(10):4803-4.
Rishmawi N, Ghneim R, Kattan R, Ghneim R, Zoughbi M, Abu-Diab A, et al. Survival of fastidious and nonfastidious aerobic bacteria in three bacterial transport swab systems. J Clin Microbiol 2007;45(4):1278-83.
Van Horn KG, Audette CD, Sebeck D, Tucker KA. Comparison of the Copan ESwab system with two Amies agar swab transport systems for maintenance of microorganism viability. J Clin Microbiol 2008;46(5):1655-8.
Van Horn KG, Rankin I. Evaluation and comparison of two Stuart's liquid swab transport systems tested by the CLSI M40 method. Eur J Clin Microbiol Infect Dis 2007;26(8):583-6.
Betters J, Karavis M, Redmond B, Dorsey R, Angelini D, Williams K, et al. Technical evaluation of sample-processing, collection, and preservation methods. Aberdeen Proving Ground, MD: U.S. Army Edgewood Chemical Biological Center, 2014;ECBC-TR-1237.
Hubbard K, Pellar G, Emanuel P. Suitability of commercial transport media for biological pathogens under nonideal conditions. Int J Microbiol 2011;2011:463096.
Army US. Medical Research Institute of Infectious Diseases. USAMRIID's medical management of biological casualties, 8th edn. Washington, DC: U.S: Government Publishing Office, 2014.
Franco-Duarte R, Cernakova L, Kadam S, Kaushik KS, Salehi B, Bevilacqua A, et al. Advances in chemical and biological methods to identify microorganisms-from past to present. Microorganisms 2019;7(5):130.
Henchal EA, Ludwig GV, Whitehouse CA, Scherer JM. Laboratory identification of biological threats. In: Dembeck ZF, editor. Medical aspects of biological warfare. Washington, DC: Office of The Surgeon General, 2007;391-414.
American Society of Microbiology. Sentinel level clinical laboratory guidelines for suspected agents of bioterrorism and emerging infectious diseases, 2018. https://www.asm.org/ASM/media/Policy-and-Advocacy/Biosafety_Sentinel_Guideline_October_2018_FINAL.pdf (accessed January 6, 2019).
Sanderson WT, Stoddard RR, Echt AS, Piacitelli CA, Kim D, Horan J, et al. Bacillus anthracis contamination and inhalational anthrax in a mail processing and distribution center. J Appl Microbiol 2004;96(5):1048-56.
Afshinnekoo E, Meydan C, Chowdhury S, Jaroudi D, Boyer C, Bernstein N, et al. Geospatial resolution of human and bacterial diversity with city-scale metagenomics. Cell Syst 2015;1(1):72-87.
Ackelsberg J, Rakeman J, Hughes S, Petersen J, Mead P, Schriefer M, et al. Lack of evidence for plague or anthrax on the New York City Subway. Cell Syst 2015;1(1):4-5.
Angelini DJ, Harris JV, Burton LL, Rastogi PR, Smith LS, Rastogi VK. Evaluation of commercial-off-the-shelf materials for the preservation of Bacillus anthracis vegetative cells for forensic analysis. J Forensic Sci 2018;63(2):412-9.
Rastogi R, Smith L, Angelini D, Harris J, Burton L, Rastogi P, et al. Evaluation of commercial off-the-shelf solutions for supporting viability retention of Yersinia pestis cells. Aberdeen Proving Ground, MD: U.S. Army Research, Development and Engineering Command, 2017;ECBC-TR-1481.
Bearden SW, Perry RD. Laboratory maintenance and characterization of Yersinia pestis. Curr Protoc Microbiol 2008;Chapter 5: Unit 5B 1.
Eisen RJ, Vetter SM, Holmes JL, Bearden SW, Montenieri JA, Gage KL. Source of host blood affects prevalence of infection and bacterial loads of Yersinia pestis in fleas. J Med Entomol 2008;45(5):933-8.
Rastogi VK, Ryan SP, Wallace L, Smith LS, Shah SS, Martin GB. Systematic evaluation of the efficacy of chlorine dioxide in decontamination of building interior surfaces contaminated with anthrax spores. Appl Environ Microbiol 2010;76(10):3343-51.
Buttner MP, Cruz P, Stetzenbach LD, Klima-Comba AK, Stevens VL, Emanuel PA. Evaluation of the Biological Sampling Kit (BiSKit) for large-area surface sampling. Appl Environ Microbiol 2004;70(12):7040-5.
U.S. Centers for Disease Control & Prevention. Surface sampling procedures for Bacillus anthracis spores from smooth, non-porous surfaces, 2012. http://www.cdc.gov/niosh/topics/emres/surface-sampling-bacillus-anthracis.html (accessed October 1, 2015).
Hutchison JR, Brooks SM, Kennedy ZC, Pope TR, Deatherage Kaiser BL, Victry KD, et al. Polysaccharide-based liquid storage and transport media for non-refrigerated preservation of bacterial pathogens. PLoS ONE 2019;14(9):e0221831.