Plasmodium infection and its association with biochemical and haematological parameters in free-living Alouatta guariba clamitans (Cabrera, 1940) (Primates: Atelidae) in Southern Brazil.
Journal
Memorias do Instituto Oswaldo Cruz
ISSN: 1678-8060
Titre abrégé: Mem Inst Oswaldo Cruz
Pays: Brazil
ID NLM: 7502619
Informations de publication
Date de publication:
2020
2020
Historique:
received:
19
06
2019
accepted:
09
12
2019
entrez:
6
2
2020
pubmed:
6
2
2020
medline:
7
3
2020
Statut:
epublish
Résumé
The influence of Plasmodium spp. infection in the health of Southern brown howler monkey, Alouatta guariba clamitans, the main reservoir of malaria in the Atlantic Forest, is still unknown. The aim of this study was to investigate the positivity rate of Plasmodium infection in free-living howler monkeys in an Atlantic Forest fragment in Joinville/SC and to associate the infection with clinical, morphometrical, haematological and biochemical alterations. Molecular diagnosis of Plasmodium infection in the captured monkeys was performed by Nested-polymerase chain reaction (PCR) (18S rRNA and coxI). Haematological and biochemical parameters were compared among infected and uninfected monkeys; clinical and morphometrical parameters were also compared. The positivity rate of Plasmodium infection was 70% among forty captured animals, the highest reported for neotropical primates. None statistical differences were detected in the clinical parameters, and morphometric measures comparing infected and uninfected groups. The main significant alteration was the higher alanine aminotransferase (ALT) levels in infected compared to uninfected monkeys. Therefore, Plasmodium infection in howler monkeys may causes haematological/biochemical alterations which might suggest hepatic impairment. Moreover, infection must be monitored for the eco-epidemiological surveillance of malaria in the Atlantic Forest and during primate conservation program that involves the animal movement, such as translocations.
Sections du résumé
BACKGROUND
BACKGROUND
The influence of Plasmodium spp. infection in the health of Southern brown howler monkey, Alouatta guariba clamitans, the main reservoir of malaria in the Atlantic Forest, is still unknown.
OBJECTIVES
OBJECTIVE
The aim of this study was to investigate the positivity rate of Plasmodium infection in free-living howler monkeys in an Atlantic Forest fragment in Joinville/SC and to associate the infection with clinical, morphometrical, haematological and biochemical alterations.
METHODS
METHODS
Molecular diagnosis of Plasmodium infection in the captured monkeys was performed by Nested-polymerase chain reaction (PCR) (18S rRNA and coxI). Haematological and biochemical parameters were compared among infected and uninfected monkeys; clinical and morphometrical parameters were also compared.
FINDINGS
RESULTS
The positivity rate of Plasmodium infection was 70% among forty captured animals, the highest reported for neotropical primates. None statistical differences were detected in the clinical parameters, and morphometric measures comparing infected and uninfected groups. The main significant alteration was the higher alanine aminotransferase (ALT) levels in infected compared to uninfected monkeys.
MAIN CONCLUSIONS
CONCLUSIONS
Therefore, Plasmodium infection in howler monkeys may causes haematological/biochemical alterations which might suggest hepatic impairment. Moreover, infection must be monitored for the eco-epidemiological surveillance of malaria in the Atlantic Forest and during primate conservation program that involves the animal movement, such as translocations.
Identifiants
pubmed: 32022168
pii: S0074-02762019000100355
doi: 10.1590/0074-02760190210
pmc: PMC6996493
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e190210Références
Malar J. 2017 Feb 10;16(1):71
pubmed: 28187764
PLoS One. 2017 Sep 13;12(9):e0184504
pubmed: 28902879
J Med Primatol. 2011 Dec;40(6):392-400
pubmed: 21933192
Sci Rep. 2018 Jan 08;8(1):86
pubmed: 29311638
Trends Parasitol. 2015 May;31(5):212-21
pubmed: 25814004
PLoS One. 2014 Oct 02;9(10):e109246
pubmed: 25275496
EBioMedicine. 2015 Jul 29;2(9):1186-92
pubmed: 26501116
Parasitol Int. 2000 Sep;49(3):219-29
pubmed: 11426577
Mem Inst Oswaldo Cruz. 2014 Aug;109(5):641-53
pubmed: 25099335
Rev Inst Med Trop Sao Paulo. 1969 Sep-Oct;11(5):299-305
pubmed: 4982318
Bull World Health Organ. 1964;31:752-3
pubmed: 14278011
Malar J. 2015 Feb 18;14:81
pubmed: 25889933
Infect Genet Evol. 2011 Jan;11(1):209-21
pubmed: 20849978
Rev Sci Tech. 2010 Aug;29(2):329-50
pubmed: 20919586
Rev Soc Bras Med Trop. 2008 Jul-Aug;41(4):386-9
pubmed: 18853012
Folia Primatol (Basel). 1991;57(2):70-82
pubmed: 1786909
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6196-200
pubmed: 2762322
Parasitology. 1985 Jun;90 ( Pt 3):573-8
pubmed: 3925423
Lancet Glob Health. 2017 Oct;5(10):e1038-e1046
pubmed: 28867401
Mem Inst Oswaldo Cruz. 2011 Aug;106 Suppl 1:52-63
pubmed: 21881757
Mol Biochem Parasitol. 1993 Apr;58(2):283-92
pubmed: 8479452
Malar J. 2007 Sep 19;6:127
pubmed: 17880709
Mem Inst Oswaldo Cruz. 1992;87 Suppl 3:1-20
pubmed: 1343676