Performance Characteristics of a Multiplex Flow Immunoassay for Detection of IgG-Class Antibodies to Measles, Mumps, Rubella, and Varicella-Zoster Viruses in Presumptively Immune Health Care Workers.
MMRV
multiplex immunoflow assay
serology
Journal
Journal of clinical microbiology
ISSN: 1098-660X
Titre abrégé: J Clin Microbiol
Pays: United States
ID NLM: 7505564
Informations de publication
Date de publication:
25 03 2020
25 03 2020
Historique:
received:
22
01
2020
accepted:
31
01
2020
pubmed:
7
2
2020
medline:
24
6
2021
entrez:
7
2
2020
Statut:
epublish
Résumé
Immunity to measles, mumps, rubella, and varicella-zoster viruses (VZV; MMRV) is a common condition of employment for health care workers (HCWs) to ensure compliance with national standards and state laws. When documentation of complete vaccination or laboratory-confirmed infection is not available, Advisory Committee on Immunization Practices (ACIP) criteria are used to guide vaccination or anti-MMRV IgG testing. We assessed the performance of the BioPlex 2200 MMRV IgG multiplex flow immunoassay (MFI; Bio-Rad Laboratories, Hercules, CA) and matched immunofluorescence assays (IFAs; MBL Bion, Des Plaines, IL) in 220 HCWs categorized by ACIP criteria for presumptive immunity to MMRV. Among HCWs presumptively immune to measles, mumps, rubella, and VZV, the Bio-Rad MFI was positive in 77.3, 85.4, 84.3, and 91.1% of HCWs, respectively. Comparatively, the Bion IFA was positive in 92.9, 91.1, and 93.5% of HCWs presumptively immune to measles, mumps, and VZV (a rubella IFA was unavailable). Among HCWs fully vaccinated against measles, mumps, and VZV, Bio-Rad MFI/Bion IFA positivity rates were 77.4%/93%, 84.8%/90.7%, and 54.5%/90.9%, respectively. The Bio-Rad MFI was positive in 83.7% of HCWs fully vaccinated against rubella. For HCWs whose last vaccination event occurred within 15 years of enrollment, 83.3, 93.3, and 74.2% were positive by the Bio-Rad measles, mumps, and rubella IgG MFIs, respectively. We show significantly decreased Bio-Rad MFI sensitivity for detection of anti-measles and anti-mumps IgG-class antibodies in presumptively immune or fully vaccinated HCWs. Although negative results typically prompt revaccination, failure to recognize presumptive immunity in individuals unable to receive live, attenuated vaccines may have employment implications.
Identifiants
pubmed: 32024728
pii: JCM.00136-20
doi: 10.1128/JCM.00136-20
pmc: PMC7098756
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin G
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 American Society for Microbiology.
Références
J Infect Dis. 2009 Feb 15;199(4):552-60
pubmed: 19113988
J Clin Virol. 2015 Aug;69:214-8
pubmed: 26209410
MMWR Recomm Rep. 2011 Nov 25;60(RR-7):1-45
pubmed: 22108587
Clin Vaccine Immunol. 2017 Jul 5;24(7):
pubmed: 28490424
Clin Infect Dis. 2014 May;58(9):1205-10
pubmed: 24585562
Arch Pediatr Adolesc Med. 2007 Mar;161(3):294-301
pubmed: 17339511
J Infect Dis. 2009 Sep 15;200(6):888-99
pubmed: 19659440
J Infect Dis. 2008 Mar 1;197 Suppl 2:S147-51
pubmed: 18419389
Am J Clin Pathol. 1996 Aug;106(2):170-4
pubmed: 8712168
J Clin Microbiol. 2005 Sep;43(9):4847-51
pubmed: 16145156
J Clin Microbiol. 1995 Apr;33(4):811-5
pubmed: 7790442
J Virol Methods. 2014 May;200:10-4
pubmed: 24486923
Clin Vaccine Immunol. 2011 Sep;18(9):1524-6
pubmed: 21795463
Clin Microbiol Rev. 2016 Jan;29(1):163-74
pubmed: 26607813
J Infect Dis. 2008 Apr 1;197(7):950-6
pubmed: 18419470
Clin Vaccine Immunol. 2017 Jan 5;24(1):
pubmed: 27852634
MMWR Recomm Rep. 2013 Jun 14;62(RR-04):1-34
pubmed: 23760231
J Infect Dis. 1990 Nov;162(5):1036-42
pubmed: 2230231
Pediatr Infect Dis J. 2014 Sep;33(9):992
pubmed: 25361027
MMWR Morb Mortal Wkly Rep. 2007 Jan 12;56(1):1-4
pubmed: 17218934
Clin Vaccine Immunol. 2013 Mar;20(3):443-5
pubmed: 23345583
Pediatrics. 2009 Dec;124(6):1633-41
pubmed: 19933727