Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease.


Journal

Hepatology communications
ISSN: 2471-254X
Titre abrégé: Hepatol Commun
Pays: United States
ID NLM: 101695860

Informations de publication

Date de publication:
Feb 2020
Historique:
received: 10 06 2019
accepted: 20 10 2019
entrez: 7 2 2020
pubmed: 7 2 2020
medline: 7 2 2020
Statut: epublish

Résumé

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer deaths worldwide. The high mortality rate in HCC is largely due to the difficulty of early detection. In this study, to improve patient outcomes, serum samples from 345 patients with HCC, 46 patients with chronic hepatitis (CH), 93 patients with liver cirrhosis (LC), and 1,033 healthy individuals were analyzed with microRNA (miRNA) microarrays. We investigated the diagnostic potential of circulating miRNAs in serum and developed a detection model of HCC, including early stage. A diagnostic model was constructed based on the expression levels of a combination of miRNAs in a discovery set. We selected 52 miRNAs that had altered expressions according to disease progression status, established the diagnostic model with a combination of eight miRNAs in the discovery set, and tested the model in a validation set. The diagnostic values for discriminating cancer from HCC at-risk control samples were as follows: area under the curve, 0.99; sensitivity, 97.7%; specificity, 94.7%. With this model, 98% of stage I HCC cases were detected; these results were much better than those observed from conventional methods.

Identifiants

pubmed: 32025611
doi: 10.1002/hep4.1451
pii: HEP41451
pmc: PMC6996324
doi:

Types de publication

Journal Article

Langues

eng

Pagination

284-297

Informations de copyright

© 2019 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

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Auteurs

Yusuke Yamamoto (Y)

Division of Molecular and Cellular Medicine National Cancer Center Research Institute Tokyo Japan.

Shunsuke Kondo (S)

Department of Hepatobiliary and Pancreatic Oncology National Cancer Center Hospital Tokyo Japan.

Juntaro Matsuzaki (J)

Division of Molecular and Cellular Medicine National Cancer Center Research Institute Tokyo Japan.

Minoru Esaki (M)

Department of Hepatobiliary and Pancreatic Surgery National Cancer Center Hospital Tokyo Japan.

Takuji Okusaka (T)

Department of Hepatobiliary and Pancreatic Oncology National Cancer Center Hospital Tokyo Japan.

Kazuaki Shimada (K)

Department of Hepatobiliary and Pancreatic Surgery National Cancer Center Hospital Tokyo Japan.

Yoshiki Murakami (Y)

Department of Hepatology Graduate School of Medicine Osaka City University Osaka Japan.
Department of Molecular Pathology Tokyo Medical University Tokyo Japan.

Masaru Enomoto (M)

Department of Hepatology Graduate School of Medicine Osaka City University Osaka Japan.

Akihiro Tamori (A)

Department of Hepatology Graduate School of Medicine Osaka City University Osaka Japan.

Ken Kato (K)

Department of Gastrointestinal Oncology National Cancer Center Hospital Tokyo Japan.

Yoshiaki Aoki (Y)

Dynacom Co., Ltd. World Business Garden E25 Chiba Japan.

Satoko Takizawa (S)

Division of Molecular and Cellular Medicine National Cancer Center Research Institute Tokyo Japan.
Toray Industries, Inc. Kamakura Japan.

Hiromi Sakamoto (H)

Department of Biobank and Tissue Resources National Cancer Center Research Institute Tokyo Japan.

Shumpei Niida (S)

Medical Genome Center National Center for Geriatrics and Gerontology Obu Japan.

Fumitaka Takeshita (F)

Fundamental Innovative Oncology Core Center National Cancer Center Research Institute Tokyo Japan.

Takahiro Ochiya (T)

Division of Molecular and Cellular Medicine National Cancer Center Research Institute Tokyo Japan.
Department of Molecular and Cellular Medicine, Institute of Medical Science Tokyo Medical University Tokyo Japan.

Classifications MeSH