Differences between muscle from osteoporotic and osteoarthritic subjects: in vitro study by diffusion-tensor MRI and histological findings.
DTI
IMCL
Osteoarthritis
Osteoporosis
Skeletal muscle
Journal
Aging clinical and experimental research
ISSN: 1720-8319
Titre abrégé: Aging Clin Exp Res
Pays: Germany
ID NLM: 101132995
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
17
08
2019
accepted:
13
01
2020
pubmed:
7
2
2020
medline:
26
11
2020
entrez:
7
2
2020
Statut:
ppublish
Résumé
Osteoarthritis and osteoporosis are strongly coupled with alterations of muscles quality and fats metabolism. However, there are no studies for investigating possible differences between osteoporotic and osteoarthritic muscles. Understanding muscle-bone and muscle-cartilage interactions would be of high clinical value. Investigate potential microstructural and physiological differences between osteoporotic and osteoarthritic muscles by diffusion Nuclear Magnetic Resonance (NMR) imaging (diffusion MRI) and histological findings. Vastus-lateralis muscles excised from osteoporotic (n = 26, T Score < - 2.5, Kellgren-Lawrence ≤ 2) and osteoarthritic (n = 26, T Score > - 2.5, Kellgren--Lawrence 3 and 4) age-matched women were investigated by NMR relaxometry, diffusion-tensor imaging (DTI) at 9.4 T, and histological techniques. Intramyocellular (IMCL) and extramyocellular (EMCL) lipid were quantified. The percentage and mean diameters of fibers I and II were evaluated. Relationship between mean diffusivity (MD), fractional anisotropy (FA), the DTI eigenvalues (λ Osteoporotic muscles showed higher MD, λ This work highlights differences between muscles of osteoporotic and osteoarthritic subjects that can be quantified by NMR DTI investigations.
Sections du résumé
BACKGROUND
BACKGROUND
Osteoarthritis and osteoporosis are strongly coupled with alterations of muscles quality and fats metabolism. However, there are no studies for investigating possible differences between osteoporotic and osteoarthritic muscles. Understanding muscle-bone and muscle-cartilage interactions would be of high clinical value.
AIM
OBJECTIVE
Investigate potential microstructural and physiological differences between osteoporotic and osteoarthritic muscles by diffusion Nuclear Magnetic Resonance (NMR) imaging (diffusion MRI) and histological findings.
METHODS
METHODS
Vastus-lateralis muscles excised from osteoporotic (n = 26, T Score < - 2.5, Kellgren-Lawrence ≤ 2) and osteoarthritic (n = 26, T Score > - 2.5, Kellgren--Lawrence 3 and 4) age-matched women were investigated by NMR relaxometry, diffusion-tensor imaging (DTI) at 9.4 T, and histological techniques. Intramyocellular (IMCL) and extramyocellular (EMCL) lipid were quantified. The percentage and mean diameters of fibers I and II were evaluated. Relationship between mean diffusivity (MD), fractional anisotropy (FA), the DTI eigenvalues (λ
RESULTS
RESULTS
Osteoporotic muscles showed higher MD, λ
CONCLUSION
CONCLUSIONS
This work highlights differences between muscles of osteoporotic and osteoarthritic subjects that can be quantified by NMR DTI investigations.
Identifiants
pubmed: 32026431
doi: 10.1007/s40520-020-01483-6
pii: 10.1007/s40520-020-01483-6
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2489-2499Subventions
Organisme : Agenzia Spaziale Italiana
ID : n. DC-DTE-2011-033
Références
WHO Study Group (2003) Prevention and management of osteoporosis. Report of a WHO Study Group WHO Tec Rep Ser 921:1–204
Felson DT, Lawrence RC, Dieppe PA et al (2000) Osteoarthritis: new insights. Part 1: the disease and its risk factors. Ann Intern Med 133:635–646
pubmed: 11033593
Yeung DKW, Griffith JF, Antonio GE et al (2005) Osteoporosis is associated with increased marrow fat content and decreased marrow fat unsaturation: a proton MR spectroscopy study. J Magn Reson Imaging 2005:279–285
Griffith JF, Yeung DKW, Antonio GE et al (2006) Vertebral marrow fat content and diffusion and perfusion indexes in women with varying bone density: MR evaluation. Radiology 241:831–838
pubmed: 17053202
Bermeo S, Gunaratnam K, Duque G (2014) Fat and bone interactions. Curr Osteoporos Rep 12:235–242
pubmed: 24599601
Schwartz AV (2015) Marrow fat and bone: review of clinical findings. Front Endocrinol 6:40
Di Pietro G, Capuani S, Manenti G et al (2016) Bone marrow lipid profiles from peripheral skeleton as potential biomarkers for osteoporosis: a 1H-MR spectroscopy study. Acad Radiol 23:273–283
pubmed: 26774740
Kumar D, Karampinos DC, MacLeod TD et al (2014) Quadriceps intramuscular fat fraction rather than muscle size is associated with knee osteoarthritis. Osteoarthritis Cartilage 22:226–234
pubmed: 24361743
Bonaccorsi G, Cafarelli FP, Cervellati C et al (2019) A new corrective model to evaluate TBS in obese post- menopausal women: a cross-sectional study. Aging Clin Exp Res. https://doi.org/10.1007/s40520-019-01317-0
doi: 10.1007/s40520-019-01317-0
pubmed: 31471889
Collins KH, Herzog W, MacDonald GZ et al (2018) Obesity, metabolic syndrome, and musculoskeletal disease: common inflammatory pathways suggest a central role for loss of muscle integrity. Front Physiol. 9:112. https://doi.org/10.3389/fphys.2018.00112
doi: 10.3389/fphys.2018.00112
pubmed: 29527173
pmcid: 5829464
Davison MJ, Maly MR, Adachi JD et al (2017) Relationships between fatty infiltration in the thigh and calf in women with knee osteoarthritis. Aging Clin Exp Res 29:291–299
pubmed: 26964549
Jordan GR, Loveridge N, Bell KL et al (2003) Increased femoral neck cancellous bone and connectivity in coxarthrosis (hip osteoarthritis). Bone 32:86–95
pubmed: 12584040
Capuani S, Piccirilli E, Di Pietro G et al (2013) Microstructural differences between osteoporotic and osteoarthritic femoral cancellous bone: an in vitro magnetic resonance micro-imaging investigation. Aging Clinic Experiment Res 25:51–54
Terracciano C, Celi M, Lecce D et al (2013) Differential features of muscle fiber atrophy in osteoporosis and osteoarthritis. Osteoporos Int 24:1095–1100
pubmed: 22535191
Scimeca M, Bonanno E, Piccirilli E et al (2015) Satellite cells CD44 positive drive muscle regeneration in osteoarthritis patients. Stem Cells Int 469459.
Zhang Y, Guo J, Duanmu Y et al (2019) Quantitative analysis of modified functional muscle-bone unit and back muscle density in patients with lumbar vertebral fracture in Chinese elderly men: a case-control study. Aging Clin Exp Res 31:637–644
pubmed: 30128661
Arokoski MH, Arokoski JPA, Haara M et al (2002) Hip muscle strength and muscle cross sectional area in men with and without hip osteoarthritis. J Rheumatol 29:2185–2195
pubmed: 12375331
Zhai G, Blizzard L, Srikanth V et al (2006) Correlates of knee pain in older adults: Tasmanian older adult cohort study. Arthritis Rheum. 55:264–271
pubmed: 16583417
Scott D, Blizzard L, Fell J et al (2012) Prospective study of self-reported pain, radiographic osteoarthritis, sarcopenia progression, and falls risk in community-dwelling older adults. Arthritis Care Res (Hoboken) 64:30–37
Di Monaco M, Vallero F, Di Monaco R, et al (2011) Prevalence of sarcopenia and its association with osteoporosis in 313 older women following a hip fracture. Arch. Gerontol Geriatr 52:71–74
pubmed: 20207030
Basser PJ, Pierpaoli C (2011) Microstructural and physiological features of tissues elucidated by quantitative-diffusion-tensor MRI. J Magn Reson 213:109–219
Damon BM, Ding Z, Anderson AW et al (2002) Validation of diffusion tensor MRI-based muscle fiber tracking. Magn Reson Med 48:97–104
pubmed: 12111936
Karampinos DC, King KF, Sutton BP et al (2009) Myofiber ellipticity as an explanation for transverse asymmetry of skeletal muscle diffusion MRI in vivo signal. Ann Biomed Eng 37:2532–2546
pubmed: 19763830
pmcid: 6617521
Galbán CJ, Maderwald S, Uffmann K et al (2004) Diffusive sensitivity to muscle architecture: a magnetic resonance diffusion tensor imaging study of the human calf. Eur J Appl Physiol 93:253–262
pubmed: 15322853
Galbán CJ, Maderwald S, Uffmann K et al (2005) A diffusion tensor imaging analysis of gender differences in water diffusivity within human skeletal muscle. NMR Biomed 18:489–498
pubmed: 16075414
McMillan AB, Shi D, Pratt SJP et al (2011) Diffusion tensor MRI to assess damage in healthy and dystrophic skeletal muscle after lengthening contractions. J Biomed Biotechnol 970726.
Sigmund EE, Sui D, Ukpebor O et al (2013) Stimulated echo diffusion tensor imaging and SPAIR T2-weighted imaging in chronic exertional compartment syndrome of the lower leg muscles. J Magn Reson Imaging 38:1073–1082
pubmed: 23440764
Heemskerk AM, Strijkers GJ, Drost MR et al (2007) Skeletal muscle degeneration and regeneration after femoral artery ligation in mice: monitoring with diffusion MR imaging. Radiology 243:413–421
pubmed: 17384238
Kanis JA, Glüer CC (2000) An update on the diagnosis and assessment of osteoporosis with densitometry. Committee of Scientific Advisors, International Osteoporosis Foundation. Osteoporos Int 11:192–202
pubmed: 10824234
Kanis JA (2002) Diagnosis of osteoporosis and assessment of fracture risk. Lancet 359:1929–1936
pubmed: 12057569
Kellgren J, Lawrence J (1957) Radiological assessment of osteo-arthrosis. Ann Rheum Dis 16:494–502
pubmed: 13498604
pmcid: 13498604
Dubowitz V, Sewry CA, Lane RJ (2007) Muscle biopsy: a practical approach. Elsevier Health Sciences.
Gouzi F, Maury J, Molinari N (2013) Reference values for vastus lateralis fiber size and type in healthy subjects over 40 years old: a systematic review and metaanalysis. J Appl Physiol 115:346–354
pubmed: 23558383
Scheel M, von Roth P, Winkler T (2013) Fiber type characterization in skeletal muscle by diffusion tensor imaging. NMR Biomed 26:1220–1224
pubmed: 23553895
Scimeca M, Orlandi A, Terrenato I et al (2014) Assessment of metal contamination in a non-small cell lung cancer by EDX microanalysis. Eur J Histochem 58:2403
pubmed: 25308844
pmcid: 4194392
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
pubmed: 13986422
pmcid: 2106263
De Santis S, Rebuzzi M, Di Pietro G et al (2010) In vitro and in vivo MR evaluation of internal gradient to assess trabecular bone density. Phys Med Biol 55:5767–5785
pubmed: 20844335
Xiao L, Wu EX (2011) Diffusion-weighted magnetic resonance spectroscopy: a novel approach to investigate intramyocellular lipids. Magn Reson Med 66:937–944
pubmed: 21928357
Steidle G, Eibofner F, Schick F (2011) Quantitative diffusion imaging of adipose tissue in the human lower leg at 1.5 T Magn Reson Med 65:1118–1124.
Weber MA (ed) (2014) Magnetic resonance imaging of the skeletal musculature. Springer, Heidelberg
Galbán CJ, Maderwald S, Stock F et al (2007) Age-related changes in skeletal muscle as detected by diffusion tensor magnetic resonance imaging. J Gerontol A Biol Sci Med Sci 62:453–458
pubmed: 17452742
Zhang J, Zhang G, Morrison B (2008) Magnetic resonance imaging of mouse skeletal muscle to measure denervation atrophy. Exp Neurol 212:448–457
pubmed: 18571650
pmcid: 2532826
Milner DJ, Mavroidis M, Weisleder N et al (2000) Desmin cytoskeleton linked to muscle mitochondrial distribution and respiratory function. J Cell Biol 150:1283–1298
pubmed: 10995435
pmcid: 2150713
Blanco FJ, Rego I, Ruiz-Romero C (2011) The role of mitochondria in osteoarthritis. Nat Rev Rheumatol 7:161–169
pubmed: 21200395
Fulle S, Protasi F, Di Tano G et al (2004) The contribution of reactive oxygen species to sarcopenia and muscle ageing. Exp Gerontol 39:17–24
pubmed: 14724060
Barja G (2014) The mitochondrial free radical theory of aging. Prog Mol Biol Transl Sci 127:1–27
pubmed: 25149212
Marzetti E, Calvani R, Cesari M et al (2013) Mitochondrial dysfunction and sarcopenia of aging: from signalling pathways to clinical trials. Int J Biochem Cell Biol 45:2288–2301
pubmed: 23845738
pmcid: 3759621
Scimeca M, Salustri A, Bonanno E et al (2017) Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis. Cell Death Dis 8:e3125
pubmed: 29022895
pmcid: 5682679
Appell HJ (1990) Muscular atrophy following immobilization: a review. Sports Med 10:42–45
pubmed: 2197699
Bougea A, Papadimas G, Papadopoulos C et al (2016) An Age-related morphometric profile of skeletal muscle in healthy untrained women. J Clin. Med 5:97
pmcid: 5126794
Muller FL, Song W, Jang YC et al (2007) Denervation-induced skeletal muscle atrophy is associated with increased mitochondrial ROS production. Am J Physiol Regul Integr Comp Physiol 293:R1159–R1168
pubmed: 17584954
Tryon LD, Vainshtein A, Memme JM et al (2014) Recent advances in mitochondrial turnover during chronic muscle disuse. Integr Med Res 3:161–171
pubmed: 28664093
pmcid: 5481769
Porcari P, Hall MG, Clark CA et al (2018) The effects of ageing on mouse muscle microstructure: a comparative study of time-dependent diffusion MRI and histological assessment. NMR Biomed 31:e3881
Capuani S, Manenti G, Iundusi R et al (2015) Focus on diffusion MR investigations of musculoskeletal tissue to improve osteoporosis diagnosis: a brief practical review. BioMed Research International. https://doi.org/10.1155/2015/948610
doi: 10.1155/2015/948610
pubmed: 25861652
pmcid: 4377366