A Global Screen for Assembly State Changes of the Mitotic Proteome by SEC-SWATH-MS.
SWATH-MS
cell cycle
monitoring state of proteome organization
protein complexes
size exclusion chromatography
Journal
Cell systems
ISSN: 2405-4720
Titre abrégé: Cell Syst
Pays: United States
ID NLM: 101656080
Informations de publication
Date de publication:
26 02 2020
26 02 2020
Historique:
received:
05
06
2019
revised:
08
11
2019
accepted:
10
01
2020
pubmed:
7
2
2020
medline:
25
5
2021
entrez:
7
2
2020
Statut:
ppublish
Résumé
Living systems integrate biochemical reactions that determine the functional state of each cell. Reactions are primarily mediated by proteins. In proteomic studies, these have been treated as independent entities, disregarding their higher-level organization into complexes that affects their activity and/or function and is thus of great interest for biological research. Here, we describe the implementation of an integrated technique to quantify cell-state-specific changes in the physical arrangement of protein complexes concurrently for thousands of proteins and hundreds of complexes. Applying this technique to a comparison of human cells in interphase and mitosis, we provide a systematic overview of mitotic proteome reorganization. The results recall key hallmarks of mitotic complex remodeling and suggest a model of nuclear pore complex disassembly, which we validate by orthogonal methods. To support the interpretation of quantitative SEC-SWATH-MS datasets, we extend the software CCprofiler and provide an interactive exploration tool, SECexplorer-cc.
Identifiants
pubmed: 32027860
pii: S2405-4712(20)30001-6
doi: 10.1016/j.cels.2020.01.001
pmc: PMC7042714
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
133-155.e6Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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