Roughened graphite biointerfaced with P450 liver microsomes: Surface and electrochemical characterizations.
Biointerfaces
Biosensors
Cytochrome P450
Drug assays
Electrocatalysis
Human liver microsomes
Journal
Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
26
08
2019
revised:
28
12
2019
accepted:
08
01
2020
pubmed:
7
2
2020
medline:
19
12
2020
entrez:
7
2
2020
Statut:
ppublish
Résumé
Low-cost, voltage-driven biocatalytic designs for rapid drug metabolism assay, chemical toxicity screening, and pollutant biosensing represent considerable significance for pharmaceutical, biomedical, and environmental applications. In this study, we have designed biointerfaces of human liver microsomes with various roughened, high-purity graphite disk electrodes to study electrochemical and electrocatalytic properties. Successful spectral and microscopic characterizations, direct bioelectronic communication, direct electron-transfer rates from the electrode to liver microsomal enzymes, microsomal heme-enzyme specific oxygen reduction currents, and voltage-driven diclofenac hydroxylation (chosen as the probe reaction) are presented.
Identifiants
pubmed: 32028130
pii: S0927-7765(20)30020-5
doi: 10.1016/j.colsurfb.2020.110790
pii:
doi:
Substances chimiques
Graphite
7782-42-5
Cytochrome P-450 Enzyme System
9035-51-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110790Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest There are no conflicts to declare.