Simple and visible detection of duck hepatitis B virus in ducks and geese using loop-mediated isothermal amplification.
DHBV
loop-mediated isothermal amplification
simplicity
visible detection
Journal
Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
09
08
2019
accepted:
01
10
2019
entrez:
8
2
2020
pubmed:
8
2
2020
medline:
2
6
2020
Statut:
ppublish
Résumé
In this study, loop-mediated isothermal amplification (LAMP) was used to establish a rapid, specific, and visual detection method for duck hepatitis B virus (DHBV). The design and synthesis of 4 specific LAMP primers were based on the conserved gene region of the DHBV genome, and the optimum temperature and time of the LAMP reaction were 63°C and 50 min, respectively. The LAMP assay was confirmed to be specific for DHBV detection and had the same sensitivity as the quantitative PCR assay. A visual detection method for rapid determination of results was developed using a color indicator containing phenol red and cresol red. A color change was produced based on a pH change in the reaction system, indicating a positive reaction. For the detection of samples from ducks and geese, the LAMP method has the advantages of simplicity, high sensitivity and specificity, good visibility, and low cost. Moreover, it is more practical and convenient than PCR-related assays for the clinical detection of DHBV.
Identifiants
pubmed: 32029161
pii: S0032-5791(19)58074-9
doi: 10.1016/j.psj.2019.12.024
pmc: PMC7587725
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
791-796Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
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