Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 02 2020
06 02 2020
Historique:
received:
17
07
2019
accepted:
07
11
2019
entrez:
8
2
2020
pubmed:
8
2
2020
medline:
12
11
2020
Statut:
epublish
Résumé
Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.
Identifiants
pubmed: 32029815
doi: 10.1038/s41598-019-54349-x
pii: 10.1038/s41598-019-54349-x
pmc: PMC7005178
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1942Références
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