Vagococcus salmoninarum II-qPCR, tropism and egg-associated transmission.
Vagococcus salmoninarum
coldwater streptococcosis
egg-associated transmission
qPCR
tissue tropism
Journal
Journal of fish diseases
ISSN: 1365-2761
Titre abrégé: J Fish Dis
Pays: England
ID NLM: 9881188
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
08
10
2019
revised:
18
11
2019
accepted:
19
11
2019
entrez:
8
2
2020
pubmed:
8
2
2020
medline:
13
8
2020
Statut:
ppublish
Résumé
Vagococcus salmoninarum was identified as the causative agent of a chronic epizootic in broodstock "coaster" brook trout (Salvelinus fontinalis) at the Iron River National Fish Hatchery. The epizootic spanned more than a year, was unresponsive to multiple florfenicol treatments, and resulted in >50% mortality of the affected fish. The decision was made to cull the remaining fish during spawning, which presented an opportunity to more thoroughly examine V. salmoninarum sampling methods, organ tropism and vertical transmission. A newly developed qPCR targeting the pheS gene was used in concert with bacterial culture to show that V. salmoninarum indeed disproportionately affects females and has a tropism for female reproductive tissues. The study demonstrates that some female reproductive tissues (e.g. ovarian fluid, unfertilized eggs) are also an effective option for non-lethal detection. Despite the widespread presence of V. salmoninarum in ovarian fluid and on egg surfaces, we found no evidence of intra-ova transmission.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
317-325Informations de copyright
© 2019 John Wiley & Sons Ltd.
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