Adherens junction remodelling during mitotic rounding of pseudostratified epithelial cells.
E-cadherin
cortex
mitosis
myosin
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
03 04 2020
03 04 2020
Historique:
received:
18
11
2019
revised:
10
12
2019
accepted:
15
01
2020
pubmed:
8
2
2020
medline:
28
4
2021
entrez:
8
2
2020
Statut:
ppublish
Résumé
Epithelial cells undergo cortical rounding at the onset of mitosis to enable spindle orientation in the plane of the epithelium. In cuboidal epithelia in culture, the adherens junction protein E-cadherin recruits Pins/LGN/GPSM2 and Mud/NuMA to orient the mitotic spindle. In the pseudostratified columnar epithelial cells of Drosophila, septate junctions recruit Mud/NuMA to orient the spindle, while Pins/LGN/GPSM2 is surprisingly dispensable. We show that these pseudostratified epithelial cells downregulate E-cadherin as they round up for mitosis. Preventing cortical rounding by inhibiting Rho-kinase-mediated actomyosin contractility blocks downregulation of E-cadherin during mitosis. Mitotic activation of Rho-kinase depends on the RhoGEF ECT2/Pebble and its binding partners RacGAP1/MgcRacGAP/CYK4/Tum and MKLP1/KIF23/ZEN4/Pav. Cell cycle control of these Rho activators is mediated by the Aurora A and B kinases, which act redundantly during mitotic rounding. Thus, in Drosophila pseudostratified epithelia, disruption of adherens junctions during mitosis necessitates planar spindle orientation by septate junctions to maintain epithelial integrity.
Identifiants
pubmed: 32030856
doi: 10.15252/embr.201949700
pmc: PMC7132200
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e49700Subventions
Organisme : UK Research and Innovation|Medical Research Council (MRC)
ID : FC0010180
Pays : International
Organisme : Wellcome Trust (WT)
ID : FC0010180
Pays : International
Organisme : Cancer Research UK
ID : FC0010180
Pays : United Kingdom
Organisme : Francis Crick Institute
ID : FC0010180
Pays : International
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : The Australian National University (ANU)
ID : R42700.11
Pays : International
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
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