Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML.
Journal
Lab on a chip
ISSN: 1473-0189
Titre abrégé: Lab Chip
Pays: England
ID NLM: 101128948
Informations de publication
Date de publication:
03 03 2020
03 03 2020
Historique:
pubmed:
8
2
2020
medline:
22
6
2021
entrez:
8
2
2020
Statut:
ppublish
Résumé
We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.
Identifiants
pubmed: 32031548
doi: 10.1039/c9lc01213c
pmc: PMC7439560
mid: NIHMS1617979
doi:
Substances chimiques
Protein Kinase Inhibitors
0
Fusion Proteins, bcr-abl
EC 2.7.10.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
942-948Subventions
Organisme : NCI NIH HHS
ID : P01 CA097132
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA181595
Pays : United States
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