TGF-β3 Loaded Electrospun Polycaprolacton Fibre Scaffolds for Rotator Cuff Tear Repair: An in Vivo Study in Rats.
Animals
Biomechanical Phenomena
/ drug effects
Bone and Bones
/ drug effects
Chitosan
/ chemistry
Cicatrix
/ drug therapy
Fibrosis
/ drug therapy
Inflammation
/ drug therapy
Neutrophils
/ drug effects
Phagocytosis
/ drug effects
Polyesters
/ chemistry
Rats
Rotator Cuff
Rotator Cuff Injuries
/ therapy
Tendon Injuries
/ drug therapy
Tendons
/ drug effects
Tissue Scaffolds
Transforming Growth Factor beta3
/ administration & dosage
Wound Healing
/ drug effects
PCL
animal study
implant
protein loading
rat model
rotator tear cuff
tendon defect
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
05 Feb 2020
05 Feb 2020
Historique:
received:
16
12
2019
revised:
27
01
2020
accepted:
04
02
2020
entrez:
9
2
2020
pubmed:
9
2
2020
medline:
20
11
2020
Statut:
epublish
Résumé
Biological factors such as TGF-β3 are possible supporters of the healing process in chronic rotator cuff tears. In the present study, electrospun chitosan coated polycaprolacton (CS-g-PCL) fibre scaffolds were loaded with TGF-β3 and their effect on tendon healing was compared biomechanically and histologically to unloaded fibre scaffolds in a chronic tendon defect rat model. The biomechanical analysis revealed that tendon-bone constructs with unloaded scaffolds had significantly lower values for maximum force compared to native tendons. Tendon-bone constructs with TGF-β3-loaded fibre scaffolds showed only slightly lower values. In histological evaluation minor differences could be observed. Both groups showed advanced fibre scaffold degradation driven partly by foreign body giant cell accumulation and high cellular numbers in the reconstructed area. Normal levels of neutrophils indicate that present mast cells mediated rather phagocytosis than inflammation. Fibrosis as sign of foreign body encapsulation and scar formation was only minorly present. In conclusion, TGF-β3-loading of electrospun PCL fibre scaffolds resulted in more robust constructs without causing significant advantages on a cellular level. A deeper investigation with special focus on macrophages and foreign body giant cells interactions is one of the major foci in further investigations.
Identifiants
pubmed: 32033294
pii: ijms21031046
doi: 10.3390/ijms21031046
pmc: PMC7036781
pii:
doi:
Substances chimiques
Polyesters
0
Transforming Growth Factor beta3
0
polycaprolactone
24980-41-4
Chitosan
9012-76-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : WE 4262/6-1
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