Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 02 2020
07 02 2020
Historique:
received:
25
11
2019
accepted:
22
01
2020
entrez:
9
2
2020
pubmed:
9
2
2020
medline:
11
11
2020
Statut:
epublish
Résumé
Neutrophils are innate immune effector cells that traffic from the circulation to extravascular sites of inflammation. β2 integrins are important mediators of the processes involved in neutrophil recruitment. Although neutrophils express the cytoskeletal protein vinculin, they do not form mature focal adhesions. Here, we characterize the role of vinculin in β2 integrin-dependent neutrophil adhesion, migration, mechanosensing, and recruitment. We observe that knockout of vinculin attenuates, but does not completely abrogate, neutrophil adhesion, spreading, and crawling under static conditions. However, we also found that vinculin deficiency does not affect these behaviors in the presence of forces from fluid flow. In addition, we identify a role for vinculin in mechanosensing, as vinculin-deficient neutrophils exhibit attenuated spreading on stiff, but not soft, substrates. Consistent with these findings, we observe that in vivo neutrophil recruitment into the inflamed peritoneum of mice remains intact in the absence of vinculin. Together, these data suggest that while vinculin regulates some aspects of neutrophil adhesion and spreading, it may be dispensable for β2 integrin-dependent neutrophil recruitment in vivo.
Identifiants
pubmed: 32034208
doi: 10.1038/s41598-020-58882-y
pii: 10.1038/s41598-020-58882-y
pmc: PMC7005776
doi:
Substances chimiques
CD18 Antigens
0
Vinculin
125361-02-6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2142Subventions
Organisme : NIDCR NIH HHS
ID : F31 DE028745
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116629
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM124911
Pays : United States
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