Integrity of dural closure after autologous platelet rich fibrin augmentation: an in vitro study.
Autologous biomaterial
Dural onlays
Neurosurgery
Watertight Dural closure
Journal
Acta neurochirurgica
ISSN: 0942-0940
Titre abrégé: Acta Neurochir (Wien)
Pays: Austria
ID NLM: 0151000
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
27
11
2019
accepted:
28
01
2020
pubmed:
9
2
2020
medline:
11
11
2020
entrez:
9
2
2020
Statut:
ppublish
Résumé
Watertight closure of the dura mater is fundamental in neurosurgery. Besides the classical suturing techniques, a variety of biomaterials have been proposed as sealants. Platelet rich fibrin (PRF) is an autologous biomaterial which can readily be obtained through low-speed centrifugation of patient's own blood. It is rich in fibrin, growth factors, leucocytes and cytokines and has shown adhesive properties while promoting the physiological wound healing process. In this study, we investigated the effect of applying PRF in reinforcing the watertight dura mater closure. We created an in vitro testing device, where the watertight dura mater closure could be hydrostatically assessed. On 26 fresh harvested bovine dura maters, a standardised 20-mm incision was closed with a running suture, and the leak pressure was measured first without (primary leak pressure) and then with PRF augmentation (secondary leak pressure). The two groups of measurements have been statistically analysed with the Student's paired t test. The "running suture only group" had a leak pressure of 10.5 ± 1.2 cmH2O (mean ± SD) while the "PRF-augmented group" had a leak pressure of 47.2 ± 2.6 cm H2O. This difference was statistically significant (p < 0.001; paired t test). Autologous platelet rich fibrin augmentation reliably reinforced watertight closure of the dura mater to a > 4-fold increased leak pressure after failure of the initial standard running suture technique.
Sections du résumé
BACKGROUND
Watertight closure of the dura mater is fundamental in neurosurgery. Besides the classical suturing techniques, a variety of biomaterials have been proposed as sealants. Platelet rich fibrin (PRF) is an autologous biomaterial which can readily be obtained through low-speed centrifugation of patient's own blood. It is rich in fibrin, growth factors, leucocytes and cytokines and has shown adhesive properties while promoting the physiological wound healing process. In this study, we investigated the effect of applying PRF in reinforcing the watertight dura mater closure.
METHODS
We created an in vitro testing device, where the watertight dura mater closure could be hydrostatically assessed. On 26 fresh harvested bovine dura maters, a standardised 20-mm incision was closed with a running suture, and the leak pressure was measured first without (primary leak pressure) and then with PRF augmentation (secondary leak pressure). The two groups of measurements have been statistically analysed with the Student's paired t test.
RESULTS
The "running suture only group" had a leak pressure of 10.5 ± 1.2 cmH2O (mean ± SD) while the "PRF-augmented group" had a leak pressure of 47.2 ± 2.6 cm H2O. This difference was statistically significant (p < 0.001; paired t test).
CONCLUSIONS
Autologous platelet rich fibrin augmentation reliably reinforced watertight closure of the dura mater to a > 4-fold increased leak pressure after failure of the initial standard running suture technique.
Identifiants
pubmed: 32034495
doi: 10.1007/s00701-020-04254-4
pii: 10.1007/s00701-020-04254-4
pmc: PMC8349340
doi:
Substances chimiques
Biocompatible Materials
0
Fibrin Tissue Adhesive
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
737-743Commentaires et corrections
Type : ErratumIn
Références
Acta Neurochir (Wien). 2011 Dec;153(12):2465-72
pubmed: 21989779
Spine (Phila Pa 1976). 2005 Jul 1;30(13):E347-51
pubmed: 15990651
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e37-44
pubmed: 16504849
Oper Neurosurg (Hagerstown). 2018 Oct 1;15(4):425-432
pubmed: 29281065
Transplant Proc. 2019 Jan - Feb;51(1):215-219
pubmed: 30655152
Med Sci Sports Exerc. 2003 Oct;35(10):1648-52
pubmed: 14523300
Surg Neurol Int. 2016 Jan 25;7(Suppl 3):S77-82
pubmed: 26904371
Neurol Res. 2014 Oct;36(10):866-72
pubmed: 24601724
J Oral Implantol. 2018 Dec;44(6):471-492
pubmed: 29870308
PLoS One. 2016 Apr 27;11(4):e0151533
pubmed: 27119993
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e56-60
pubmed: 16504852
Acta Neurochir (Wien). 2018 Aug;160(8):1497-1503
pubmed: 29872915
Muscles Ligaments Tendons J. 2014 May 08;4(1):3-9
pubmed: 24932440
Eur J Trauma Emerg Surg. 2018 Feb;44(1):87-95
pubmed: 28283682
Clin Oral Investig. 2016 Dec;20(9):2353-2360
pubmed: 26809431
Skull Base Surg. 1997;7(1):23-30
pubmed: 17171003
Biomaterials. 2007 Nov;28(31):4551-60
pubmed: 17659771
J Clin Neurosci. 2003 Jan;10(1):92-5
pubmed: 12464532
J Extra Corpor Technol. 2006 Jun;38(2):174-87
pubmed: 16921694
Rev Assoc Med Bras (1992). 2018 Dec;64(12):1069-1072
pubmed: 30569979
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e45-50
pubmed: 16504850
Surg Neurol Int. 2015 May 12;6:77
pubmed: 25984391
Tissue Eng Part A. 2011 Feb;17(3-4):349-59
pubmed: 20799908
J Clin Neurosci. 2003 Nov;10(6):661-4
pubmed: 14592612
Br J Neurosurg. 2015 Feb;29(1):11-17
pubmed: 25112563
Eur Spine J. 2009 Aug;18(8):1169-74
pubmed: 19283413
Acta Neurochir (Wien). 2002 Mar;144(3):265-9; discussion 269
pubmed: 11956939
Clin Orthop Relat Res. 2015 May;473(5):1635-43
pubmed: 25690170
Curr Pharm Biotechnol. 2012 Jun;13(7):1121-30
pubmed: 21740380
Curr Pharm Biotechnol. 2012 Jun;13(7):1266-77
pubmed: 21740368
J Ophthalmic Vis Res. 2019 Apr-Jun;14(2):136-143
pubmed: 31114649
J Knee Surg. 2018 Jan;31(1):99-116
pubmed: 28464195
Spine J. 2010 Dec;10(12):1065-8
pubmed: 21094467
J Neurol Surg B Skull Base. 2012 Dec;73(6):401-4
pubmed: 24294557