Inactivation of foot-and-mouth disease virus A/IRN/8/2015 with commercially available lysis buffers.
Foot-and-mouth disease virus
Lysis buffer
Virus inactivation
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
09
09
2019
revised:
03
02
2020
accepted:
04
02
2020
pubmed:
9
2
2020
medline:
29
5
2021
entrez:
9
2
2020
Statut:
ppublish
Résumé
Laboratories working with foot-and-mouth disease virus (FMDV) must maintain a high level of biocontainment. However, if infectious virus is reliably inactivated during sample processing, molecular and serological testing can be performed at a lower level of containment. In this study, three commercial lysis buffers (AL, AVL, and MagMAX CORE) were tested in two laboratories for their ability to inactivate FMDV A/IRN/8/2015 in different sample matrices (cell culture supernatant, epithelial tissue suspension and milk). Residual infectivity after the addition of lysis buffer was evaluated by inoculating susceptible cell cultures. No cytopathic effect was observed for all three lysis buffers, indicating that the buffers are capable of reducing viral infectivity (estimated range 3.1 to >5.1 Log
Identifiants
pubmed: 32035122
pii: S0166-0934(19)30402-1
doi: 10.1016/j.jviromet.2020.113835
pii:
doi:
Substances chimiques
Buffers
0
Indicators and Reagents
0
Guanidine
JU58VJ6Y3B
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113835Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no competing interests.