Artificial generation of centromeres and kinetochores to understand their structure and function.
Centromere
Chromatin
Epigenetics
Kinetochore
Neocentromere
Journal
Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226
Informations de publication
Date de publication:
15 04 2020
15 04 2020
Historique:
received:
03
12
2019
revised:
18
01
2020
accepted:
05
02
2020
pubmed:
10
2
2020
medline:
11
11
2020
entrez:
10
2
2020
Statut:
ppublish
Résumé
The centromere is an essential genomic region that provides the surface to form the kinetochore, which binds to the spindle microtubes to mediate chromosome segregation during mitosis and meiosis. Centromeres of most organisms possess highly repetitive sequences, making it difficult to study these loci. However, an unusual centromere called a "neocentromere," which does not contain repetitive sequences, was discovered in a patient and can be generated experimentally. Recent advances in genome biology techniques allow us to analyze centromeric chromatin using neocentromeres. In addition to neocentromeres, artificial kinetochores have been generated on non-centromeric loci, using protein tethering systems. These are powerful tools to understand the mechanism of the centromere specification and kinetochore assembly. In this review, we introduce recent studies utilizing the neocentromeres and artificial kinetochores and discuss current problems in centromere biology.
Identifiants
pubmed: 32035949
pii: S0014-4827(20)30097-5
doi: 10.1016/j.yexcr.2020.111898
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
111898Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.