The Effects of the Anti-aging Protein Klotho on Mucociliary Clearance.
TGF-β
airway surface liquid volume
calcium activated potassium channels
klotho
mucociliary clearance
Journal
Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047
Informations de publication
Date de publication:
2019
2019
Historique:
received:
30
08
2019
accepted:
24
12
2019
entrez:
11
2
2020
pubmed:
11
2
2020
medline:
11
2
2020
Statut:
epublish
Résumé
α-klotho (KL) is an anti-aging protein and has been shown to exert anti-inflammatory and anti-oxidative effects in the lung and pulmonary diseases such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis. The current study investigated the direct effect of KL on the bronchial epithelium in regards to mucociliary clearance parameters. Primary human bronchial and murine tracheal epithelial cells, cultured, and differentiated at the air liquid interface (ALI), were treated with recombinant KL or infected with a lentiviral vector expressing KL. Airway surface liquid (ASL) volume, airway ion channel activities, and expression levels were analyzed. These experiments were paired with
Identifiants
pubmed: 32039219
doi: 10.3389/fmed.2019.00339
pmc: PMC6992571
doi:
Types de publication
Journal Article
Langues
eng
Pagination
339Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK072482
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL160911
Pays : United States
Organisme : NIA NIH HHS
ID : R03 AG059994
Pays : United States
Organisme : NHLBI NIH HHS
ID : R35 HL135816
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL139365
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL128714
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133240
Pays : United States
Informations de copyright
Copyright © 2020 Garth, Easter, Skylar Harris, Sailland, Kuenzi, Chung, Dennis, Baumlin, Adewale, Rowe, King, Faul, Barnes, Salathe and Krick.
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