Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation.
Animals
Animals, Genetically Modified
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ embryology
Eukaryotic Initiation Factor-4E
/ genetics
Gene Expression Regulation, Developmental
Microfilament Proteins
/ genetics
Muscle Proteins
/ genetics
Muscle, Skeletal
/ embryology
Neuromuscular Junction
/ embryology
Neuronal Plasticity
RNA-Binding Proteins
/ genetics
Time Factors
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
02 03 2020
02 03 2020
Historique:
received:
22
03
2019
revised:
21
08
2019
accepted:
12
12
2019
entrez:
11
2
2020
pubmed:
11
2
2020
medline:
21
10
2020
Statut:
ppublish
Résumé
Memory and learning involve activity-driven expression of proteins and cytoskeletal reorganization at new synapses, requiring posttranscriptional regulation of localized mRNA a long distance from corresponding nuclei. A key factor expressed early in synapse formation is Msp300/Nesprin-1, which organizes actin filaments around the new synapse. How Msp300 expression is regulated during synaptic plasticity is poorly understood. Here, we show that activity-dependent accumulation of Msp300 in the postsynaptic compartment of the Drosophila larval neuromuscular junction is regulated by the conserved RNA binding protein Syncrip/hnRNP Q. Syncrip (Syp) binds to msp300 transcripts and is essential for plasticity. Single-molecule imaging shows that msp300 is associated with Syp in vivo and forms ribosome-rich granules that contain the translation factor eIF4E. Elevated neural activity alters the dynamics of Syp and the number of msp300:Syp:eIF4E RNP granules at the synapse, suggesting that these particles facilitate translation. These results introduce Syp as an important early acting activity-dependent regulator of a plasticity gene that is strongly associated with human ataxias.
Identifiants
pubmed: 32040548
pii: 133707
doi: 10.1083/jcb.201903135
pmc: PMC7055005
pii:
doi:
Substances chimiques
Drosophila Proteins
0
Eukaryotic Initiation Factor-4E
0
Microfilament Proteins
0
Msp300 protein, Drosophila
0
Muscle Proteins
0
RNA-Binding Proteins
0
Syp protein, Drosophila
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
ID : 091911
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 209412
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 107457
Pays : United Kingdom
Organisme : Medical Research Council
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 209412/Z/17/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 096144
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : P41 GM103540
Pays : United States
Organisme : Wellcome Trust
ID : 091911
Pays : United Kingdom
Informations de copyright
© 2020 Titlow et al.
Références
J Microsc. 2008 Jan;229(Pt 1):78-91
pubmed: 18173647
Biol Open. 2012 May 15;1(5):488-97
pubmed: 23213441
Nat Protoc. 2010 Nov;5(11):1761-74
pubmed: 21030952
Sci Signal. 2013 Dec 17;6(306):rs16
pubmed: 24345682
JAMA Neurol. 2013 Oct;70(10):1296-31
pubmed: 23959263
Nucleus. 2013 Jan-Feb;4(1):18-22
pubmed: 23211643
Mol Cell Neurosci. 2018 Mar;87:27-34
pubmed: 29254824
Nat Genet. 2007 Jan;39(1):80-5
pubmed: 17159980
Cell Rep. 2016 Oct 11;17(3):799-808
pubmed: 27732855
J Neurosci. 2003 Jul 23;23(16):6546-56
pubmed: 12878696
Antioxid Redox Signal. 2014 Dec 20;21(18):2483-97
pubmed: 24844655
Neuron. 2008 Mar 13;57(5):705-18
pubmed: 18341991
J Neurosci. 2002 Sep 1;22(17):7362-72
pubmed: 12196557
G3 (Bethesda). 2014 Apr 16;4(4):749-60
pubmed: 24531791
Biol Open. 2014 Aug 29;3(9):839-49
pubmed: 25171887
Science. 2000 Mar 24;287(5461):2222-4
pubmed: 10731138
RNA. 2014 Oct;20(10):1593-606
pubmed: 25171822
J Cell Sci. 2008 Mar 15;121(Pt 6):887-94
pubmed: 18303053
Nucleic Acids Res. 1998 Jan 15;26(2):655-61
pubmed: 9421530
J Cell Biol. 2000 Jan 24;148(2):353-62
pubmed: 10648568
Brain. 2016 Aug;139(Pt 8):e46
pubmed: 27197992
Mol Gen Genet. 1982;187(1):37-41
pubmed: 6819428
J Chem Neuroanat. 2017 Jan;79:12-21
pubmed: 27771350
Mol Reprod Dev. 2015 Jul-Aug;82(7-8):518-29
pubmed: 26153368
Biochem Soc Trans. 2018 Jun 19;46(3):669-681
pubmed: 29784648
Mov Disord. 2016 Nov;31(11):1754-1756
pubmed: 27671794
Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7062-8
pubmed: 11416188
Mol Cell Biol. 2012 Feb;32(3):717-28
pubmed: 22124155
Wiley Interdiscip Rev Dev Biol. 2013 Sep-Oct;2(5):647-70
pubmed: 24014452
Virol J. 2013 May 16;10:151
pubmed: 23679954
Nat Genet. 2017 Jun;49(6):866-875
pubmed: 28436985
Neuron. 2004 Nov 18;44(4):663-76
pubmed: 15541314
Cell Rep. 2017 Mar 14;18(11):2795-2806
pubmed: 28297680
Neuron. 2009 Oct 29;64(2):173-87
pubmed: 19874786
Methods Mol Biol. 2018;1649:163-175
pubmed: 29130196
Neuron. 2004 Nov 18;44(4):677-90
pubmed: 15541315
J Biol Chem. 2015 Nov 27;290(48):28613-22
pubmed: 26453304
Genetics. 2015 Oct;201(2):345-75
pubmed: 26447126
Bioinformatics. 2016 Mar 15;32(6):958-60
pubmed: 26589275
Mol Cell Biol. 2004 Sep;24(18):7878-90
pubmed: 15340051
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Biophys J. 2009 Jan;96(2):707-16
pubmed: 19167315
J Cell Sci. 2016 Jan 1;129(1):166-77
pubmed: 26567222
Development. 1992 Nov;116(3):721-30
pubmed: 1289062
Methods. 2017 Feb 15;115:28-41
pubmed: 28057586
Biochem Soc Trans. 2018 Apr 17;46(2):311-320
pubmed: 29487227
Mol Cell Biol. 2012 Jun;32(12):2224-38
pubmed: 22493061
J Neurochem. 2008 Apr;105(2):351-9
pubmed: 18045242
J Neurogenet. 2016 Sep - Dec;30(3-4):237-246
pubmed: 27981875
Biophys J. 2000 Feb;78(2):901-7
pubmed: 10653802
J Neurosci. 2003 Nov 12;23(32):10433-44
pubmed: 14614102
Curr Biol. 2017 May 8;27(9):1387-1391
pubmed: 28457866
Neuron. 2015 May 20;86(4):1015-1028
pubmed: 25959729
J Cell Biol. 2015 May 25;209(4):529-38
pubmed: 26008743
J Neurol. 2016 Aug;263(8):1503-10
pubmed: 27178001
Cell Death Differ. 2013 Feb;20(2):226-34
pubmed: 22935615
Elife. 2016 Jan 13;5:
pubmed: 26760529
Genesis. 2004 Aug;39(4):240-5
pubmed: 15286996
Mol Cell Proteomics. 2016 Feb;15(2):368-81
pubmed: 26307175
J Cell Biol. 2017 Jul 3;216(7):1915-1924
pubmed: 28533284
Mol Cell Neurosci. 2014 Jul;61:241-54
pubmed: 25066865
J Neurol Sci. 2018 Jul 15;390:227-230
pubmed: 29801895
Nat Methods. 2013 Apr;10(4):277-8
pubmed: 23538861
Proc Natl Acad Sci U S A. 2019 Jul 23;116(30):15023-15032
pubmed: 31292258
J Biol Chem. 1993 Feb 15;268(5):3017-20
pubmed: 8428975
EMBO Rep. 2017 May;18(5):693-711
pubmed: 28404606
Nature. 2000 Jun 29;405(6790):1062-5
pubmed: 10890448
Neuron. 1998 Oct;21(4):741-51
pubmed: 9808461
J Neurosci. 2000 Jun 1;20(11):3993-4001
pubmed: 10818134
Nat Neurosci. 2016 Sep;19(9):1194-6
pubmed: 27479843
Science. 2014 Jan 24;343(6169):419-22
pubmed: 24458642
Ann Neurol. 2007 Jul;62(1):93-8
pubmed: 17503513
Cold Spring Harb Perspect Biol. 2011 Jun 01;3(6):
pubmed: 21555405
Sci Rep. 2018 May 15;8(1):7583
pubmed: 29765093
Sci Rep. 2015 Nov 03;5:15915
pubmed: 26525406
Development. 2013 Dec;140(23):4818-25
pubmed: 24154526
Elife. 2016 Dec 09;5:
pubmed: 27936378
Brain. 2016 May;139(Pt 5):1378-93
pubmed: 27086870
J Biol Chem. 2004 Dec 17;279(51):53427-34
pubmed: 15475564
Development. 2014 Oct;141(20):3994-4005
pubmed: 25294943
Genetics. 2007 Mar;175(3):1505-31
pubmed: 17194782
J Cell Biol. 1996 May;133(3):495-505
pubmed: 8636226
Cell Mol Life Sci. 2014 Sep;71(17):3363-79
pubmed: 24492984