Do storage solutions protect endothelial function of arterialized vein graft in an experimental rat model?
Animals
Anticoagulants
/ administration & dosage
Aorta, Abdominal
/ surgery
Blood
Coronary Artery Bypass
Disease Models, Animal
Endothelium, Vascular
/ drug effects
Heparin
/ administration & dosage
Hyperplasia
Male
Organ Preservation Solutions
/ administration & dosage
Rats
Rats, Inbred Lew
Reactive Oxygen Species
/ analysis
Saline Solution
/ administration & dosage
Tunica Intima
/ drug effects
Vena Cava, Inferior
/ drug effects
Coronary artery bypass grafting
Storage solutions
Vascular reactivity
Venous graft
Journal
Journal of cardiothoracic surgery
ISSN: 1749-8090
Titre abrégé: J Cardiothorac Surg
Pays: England
ID NLM: 101265113
Informations de publication
Date de publication:
10 Feb 2020
10 Feb 2020
Historique:
received:
24
09
2019
accepted:
30
01
2020
entrez:
12
2
2020
pubmed:
12
2
2020
medline:
7
7
2020
Statut:
epublish
Résumé
This study aims to compare the effects of storage solutions commonly used in coronary artery bypass grafting on the vascular reactivity in vein graft interposed in arterial position in syngeneic rats. Twenty-seven male Lewis rats were sacrified to sample a vein graft implanted 6 weeks ago into abdominal aorta position. The vein grafts were inferior venae cavae initially pretreated with heparinized saline solution (HS) or autologous heparinized blood (AHB) or our referent solution, GALA. The endothelial functionality, the in situ Reactive Oxygen Species (ROS) levels and the histological characteristics were conducted from segments of arterialized vein graft. At 6 weeks, graft thrombosis occurred respectively in 22% of AHB group, 62.5% in the HS group and 82.5% in the GALA group. In each group, significative intimal hyperplasia was observed. After 6 weeks, an endothelium-remodeling layer associated with an increase of wall thickness was observed in each group. Endothelium-dependent tone was reduced in the vein graft regardless of the group. No difference was observed concerning the ROS in vein graft between the different groups. In distal aortic sections, ROS levels were increased in HS and GALA groups. Storage solutions used in this experimental model of vein graft implanted in arterial position cause graft injury and a complete disappearance of vascular reactivity. GALA solution did not reduce intimal risk hyperplasia when the vein graft was exposed to arterial flow in a rat model.
Sections du résumé
BACKGROUND
BACKGROUND
This study aims to compare the effects of storage solutions commonly used in coronary artery bypass grafting on the vascular reactivity in vein graft interposed in arterial position in syngeneic rats.
METHODS
METHODS
Twenty-seven male Lewis rats were sacrified to sample a vein graft implanted 6 weeks ago into abdominal aorta position. The vein grafts were inferior venae cavae initially pretreated with heparinized saline solution (HS) or autologous heparinized blood (AHB) or our referent solution, GALA. The endothelial functionality, the in situ Reactive Oxygen Species (ROS) levels and the histological characteristics were conducted from segments of arterialized vein graft.
RESULTS
RESULTS
At 6 weeks, graft thrombosis occurred respectively in 22% of AHB group, 62.5% in the HS group and 82.5% in the GALA group. In each group, significative intimal hyperplasia was observed. After 6 weeks, an endothelium-remodeling layer associated with an increase of wall thickness was observed in each group. Endothelium-dependent tone was reduced in the vein graft regardless of the group. No difference was observed concerning the ROS in vein graft between the different groups. In distal aortic sections, ROS levels were increased in HS and GALA groups.
CONCLUSIONS
CONCLUSIONS
Storage solutions used in this experimental model of vein graft implanted in arterial position cause graft injury and a complete disappearance of vascular reactivity. GALA solution did not reduce intimal risk hyperplasia when the vein graft was exposed to arterial flow in a rat model.
Identifiants
pubmed: 32041642
doi: 10.1186/s13019-020-1077-6
pii: 10.1186/s13019-020-1077-6
pmc: PMC7011455
doi:
Substances chimiques
Anticoagulants
0
Organ Preservation Solutions
0
Reactive Oxygen Species
0
Saline Solution
0
Heparin
9005-49-6
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
34Commentaires et corrections
Type : CommentIn
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