Anatomical and single-cell transcriptional profiling of the murine habenular complex.
VTA
dopamine
habenula
mouse
neuroscience
serotonin
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
11 02 2020
11 02 2020
Historique:
received:
22
08
2019
accepted:
21
01
2020
entrez:
12
2
2020
pubmed:
12
2
2020
medline:
7
4
2021
Statut:
epublish
Résumé
The lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of the habenula remain unknown. Here, we use high-throughput single-cell transcriptional profiling, monosynaptic retrograde tracing, and multiplexed FISH to characterize the cells of the mouse habenula. We find five subtypes of neurons in the medial habenula (MHb) that are organized into anatomical subregions. In the LHb, we describe four neuronal subtypes and show that they differentially target dopaminergic and GABAergic cells in the ventral tegmental area (VTA). These data provide a valuable resource for future study of habenular function and dysfunction and demonstrate neuronal subtype specificity in the LHb-VTA circuit.
Identifiants
pubmed: 32043968
doi: 10.7554/eLife.51271
pii: 51271
pmc: PMC7012610
doi:
pii:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R01 NS103226
Pays : United States
Organisme : NINDS NIH HHS
ID : NS105883
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
© 2020, Wallace et al.
Déclaration de conflit d'intérêts
MW, KH, DH, MH, GR, BS No competing interests declared
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