Induction of foxo3a protects turtle neurons against oxidative stress.
Epigallochatechin-3-gallate
Reactive oxygen species
Reoxygenation
Trachemys scripta
Journal
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
ISSN: 1531-4332
Titre abrégé: Comp Biochem Physiol A Mol Integr Physiol
Pays: United States
ID NLM: 9806096
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
12
10
2019
revised:
30
01
2020
accepted:
02
02
2020
pubmed:
12
2
2020
medline:
4
3
2021
entrez:
12
2
2020
Statut:
ppublish
Résumé
The detrimental effects of oxidative stress caused by the accumulation of Reactive Oxygen Species (ROS) factor into aging, senescence and several neurodegenerative diseases. Mammalian models are extremely susceptible to the stresses that follow the restoration of oxygen after anoxia; however some organisms including the freshwater turtle Trachemys scripta can withstand extended anoxia and reoxygenation without apparent pathology. The ability of the turtle to withstand these conditions is thought to be linked to the upregulation of protective mechanisms such as heat shock proteins (HSP) as well as the suppression of ROS formation and the upregulation of antioxidant defenses. One such antioxidant mechanism is the transcription factor Forkhead box O3a (FOXO3a), that has been shown to be activated in several animal models during oxidative stress. In this study, we utilized both the transfection of a plasmid carrying foxo3a and the pharmacological manipulation of foxo3a using the green tea extract Epigallocatechin-3-gallate (EGCG) to investigate the protective role of FOXO3a in the turtle brain. Our studies found that transcript levels of foxo3a were upregulated significantly during reoxygenation with greater increases during chemical oxidative stress. Induction of foxo3a by direct transfection significantly decreased cell death during chemical oxidative stress. Cells treated with EGCG also showed increased foxo3a expression and decreased cell death in the presence of H
Identifiants
pubmed: 32044446
pii: S1095-6433(20)30023-4
doi: 10.1016/j.cbpa.2020.110671
pmc: PMC8851872
mid: NIHMS1565578
pii:
doi:
Substances chimiques
Forkhead Box Protein O3
0
Neuroprotective Agents
0
Catechin
8R1V1STN48
epigallocatechin gallate
BQM438CTEL
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110671Subventions
Organisme : NIA NIH HHS
ID : R15 AG033374
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None
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