(Strept)avidin as a template for ligands other than biotin: An overview.
Avidin
Biotin
Cholic acid
Folic acid
Phage display
Progesterone
Steroid
Streptavidin
Testosterone
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
13
2
2020
pubmed:
13
2
2020
medline:
7
4
2021
Statut:
ppublish
Résumé
Chicken avidin and bacterial streptavidin are workhorses in biotechnology. We have used avidin as a scaffold protein to develop avidin variants with novel ligand-binding affinity, so-called antidins. This article covers the strategy applied in the development of antidins. Using a phage display developed for avidin, immobilized ligands were used to select binders from a phage pool displaying avidin variants with randomized sequence in the protein loops. Antidins binding various ligands with nanomolar affinity were obtained. Antidins have already been demonstrated to be suitable for a diagnostic assay measuring serum progesterone levels and they offer a promising alternative to antibodies for the recognition of small molecules.
Identifiants
pubmed: 32046847
pii: S0076-6879(19)30442-2
doi: 10.1016/bs.mie.2019.10.029
pii:
doi:
Substances chimiques
Ligands
0
Peptide Library
0
Avidin
1405-69-2
Progesterone
4G7DS2Q64Y
Biotin
6SO6U10H04
Streptavidin
9013-20-1
Hydrocortisone
WI4X0X7BPJ
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
21-28Informations de copyright
© 2020 Elsevier Inc. All rights reserved.