B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.
B cells
IgA
Peyer’s patches
SAP
T follicular helper cells
antibody
clonal diversification
germinal center
plasma cells
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
11 02 2020
11 02 2020
Historique:
received:
12
06
2019
revised:
24
10
2019
accepted:
08
01
2020
entrez:
13
2
2020
pubmed:
13
2
2020
medline:
9
3
2021
Statut:
ppublish
Résumé
Antibodies secreted within the intestinal tract provide protection from the invasion of microbes into the host tissues. Germinal center (GC) formation in lymph nodes and spleen strictly requires SLAM-associated protein (SAP)-mediated T cell functions; however, it is not known whether this mechanism plays a similar role in mucosal-associated lymphoid tissues. Here, we find that in Peyer's patches (PPs), SAP-mediated T cell help is required for promoting B cell selection in GCs, but not for clonal diversification. PPs of SAP-deficient mice host chronic GCs that are absent in T cell-deficient mice. GC B cells in SAP-deficient mice express AID and Bcl6 and generate plasma cells in proportion to the GC size. Single-cell IgA sequencing analysis reveals that these mice host few diversified clones that were subjected to mild selection forces. These findings demonstrate that T cell-derived help to B cells in PPs includes SAP-dependent and SAP-independent functions.
Identifiants
pubmed: 32049020
pii: S2211-1247(20)30047-4
doi: 10.1016/j.celrep.2020.01.032
pmc: PMC7016508
pii:
doi:
Substances chimiques
Signaling Lymphocytic Activation Molecule Associated Protein
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1910-1922.e5Subventions
Organisme : European Research Council
ID : 677713
Pays : International
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N011740/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/B/000C0427
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/B/000C0407
Pays : United Kingdom
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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