Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37.
N-acetylcysteine
acute respiratory distress syndrome
alveolar epithelial cells
bacterial lipopolysaccharide
cathelicidin LL-37
surfactant proteins
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
09 Feb 2020
09 Feb 2020
Historique:
received:
13
01
2020
revised:
02
02
2020
accepted:
07
02
2020
entrez:
14
2
2020
pubmed:
14
2
2020
medline:
21
11
2020
Statut:
epublish
Résumé
Alveolar epithelial type II (ATII) cells and their proper function are essential for maintaining lung integrity and homeostasis. However, they can be damaged by lipopolysaccharide (LPS) during Gram-negative bacterial infection. Thus, this study evaluated and compared the effects of LPS on short and long-term cultures of A549 cells by determining the cell viability, levels of oxidative stress and antimicrobial peptide cathelicidin LL-37 and changes in the expression of surfactant proteins (SPs). Moreover, we compared A549 cell response to LPS in the presence of different serum concentrations. Additionally, the effect of
Identifiants
pubmed: 32050475
pii: ijms21031148
doi: 10.3390/ijms21031148
pmc: PMC7036965
pii:
doi:
Substances chimiques
Antimicrobial Cationic Peptides
0
Lipopolysaccharides
0
Pulmonary Surfactant-Associated Proteins
0
Cathelicidins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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