Contribution of de novo and inherited rare CNVs to very preterm birth.

DNA Copy Number Variations / genetics Female Genetic Predisposition to Disease Genome-Wide Association Study Gestational Age Humans Infant, Extremely Premature / growth & development Infant, Newborn Male Pregnancy Premature Birth / genetics

copy-number developmental microarray molecular genetics

Journal

Journal of medical genetics

ISSN: 1468-6244

Titre abrégé: J Med Genet

Pays: England

ID NLM: 2985087R

Informations de publication

Date de publication:
08 2020

Historique:

received: 09 10 2019

revised: 23 12 2019

accepted: 12 01 2020

pubmed: 14 2 2020

medline: 11 6 2021

entrez: 14 2 2020

Statut: ppublish

Résumé

The genomic contribution to adverse health sequelae in babies born very preterm (<32 weeks' gestation) is unknown. We conducted an investigation of rare CNVs in infants born very preterm as part of a study to determine the feasibility and acceptability of a larger, well-powered genome-wide investigation in the UK, with follow-up using linked National Health Service records and DNA storage for additional research. We studied 488 parent-offspring trios. We performed genotyping using Illumina Infinium OmniExpress Arrays. CNV calling and quality control (QC) were undertaken using published protocols. We examined de novo CNVs in infants and the rate of known pathogenic variants in infants, mothers and fathers and compared these with published comparator data. We defined rare pathogenic CNVs as those consistently reported to be associated with clinical phenotypes. We identified 14 de novo CNVs, representing a mutation rate of 2.9%, compared with 2.1% reported in control populations. The median size of these CNV was much higher than in comparator data (717 kb vs 255 kb). The rate of pathogenic CNVs was 4.3% in infants, 2.7% in mothers and 2% in fathers, compared with 2.3% in UK Biobank participants. Our findings suggest that the rate of de novo CNVs, especially rare pathogenic CNVs, could be elevated in those born very preterm. However, we will need to conduct a much larger study to corroborate this conclusion.

Sections du résumé

BACKGROUND

METHODS

We studied 488 parent-offspring trios. We performed genotyping using Illumina Infinium OmniExpress Arrays. CNV calling and quality control (QC) were undertaken using published protocols. We examined de novo CNVs in infants and the rate of known pathogenic variants in infants, mothers and fathers and compared these with published comparator data. We defined rare pathogenic CNVs as those consistently reported to be associated with clinical phenotypes.

RESULTS

We identified 14 de novo CNVs, representing a mutation rate of 2.9%, compared with 2.1% reported in control populations. The median size of these CNV was much higher than in comparator data (717 kb vs 255 kb). The rate of pathogenic CNVs was 4.3% in infants, 2.7% in mothers and 2% in fathers, compared with 2.3% in UK Biobank participants.

CONCLUSION

Our findings suggest that the rate of de novo CNVs, especially rare pathogenic CNVs, could be elevated in those born very preterm. However, we will need to conduct a much larger study to corroborate this conclusion.

Identifiants

DOI: 10.1136/jmedgenet-2019-106619 PMID: 32051258

pubmed: 32051258

pii: jmedgenet-2019-106619

doi: 10.1136/jmedgenet-2019-106619

doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng