Tartary buckwheat extract alleviates alcohol-induced acute and chronic liver injuries through the inhibition of oxidative stress and mitochondrial cell death pathway.
Tartary buckwheat extract (TBE)
alcoholic liver injuries
mitochondrial cell death
oxidative stress
Journal
American journal of translational research
ISSN: 1943-8141
Titre abrégé: Am J Transl Res
Pays: United States
ID NLM: 101493030
Informations de publication
Date de publication:
2020
2020
Historique:
received:
31
10
2019
accepted:
18
12
2019
entrez:
14
2
2020
pubmed:
14
2
2020
medline:
14
2
2020
Statut:
epublish
Résumé
Alcohol use disorder (AUD) is an enormous public health problem that poses significant social, medical, and economic burdens. Under AUD, the liver is one of the most adversely affected organs. As current therapies and protective drugs for AUD-mediated liver injury are very limited, the prevention and therapy of alcoholic liver disease are urgently needed. The present study aims to investigate the beneficial effects of tartary buckwheat extract (TBE), the important component of Maopu tartary buckwheat liquor, on both alcoholic-induced acute and chronic liver injuries. We show that the TBE administration, similar to curcumin, significantly reduces the elevated serum aspartate aminotransferase and alanine aminotransferase levels, improves liver index, alleviates the elevated contents of hepatic malondialdehye, and restores the decreased contents of hepatic glutathione both in acute and chronic liver injuries in alcohol-exposed rats. Furthermore, histopathological analyses show that a medium dose of TBE (16.70 ml/kg body weight) alleviates hepatocyte morphology changes in both acute and chronic alcohol exposure models. We also show the protective effects of TBE on the cell death rates of alcohol-exposed primary cultured hepatocytes, HepG2 hepatoma, and Huh 7 hepatoma cells. Furthermore, we demonstrate that TBE exerts hepatoprotection partly through inhibiting the mitochondrial cell death pathway by reducing cytochrome c release, caspase-9 and -3 activities, and the number of TUNEL-positive cells. These effects of TBE were accompanied by enhanced levels of Bcl-2 and Bcl-xL and autophagic cell death pathway by reducing Beclin-1 expression, as well as through promoting its anti-oxidant capacity by suppressing reactive oxygen species production. This study demonstrates, for the first time, the protective effect of TBE against alcohol-induced acute and chronic liver injury
Types de publication
Journal Article
Langues
eng
Pagination
70-89Informations de copyright
AJTR Copyright © 2020.
Déclaration de conflit d'intérêts
None.
Références
Meat Sci. 2017 Dec;134:79-85
pubmed: 28763700
Oxid Med Cell Longev. 2018 Jun 14;2018:6712407
pubmed: 30013721
J Agric Food Chem. 2003 Oct 22;51(22):6452-5
pubmed: 14558761
Pharmacol Ther. 2018 May;185:64-85
pubmed: 29191394
Phytomedicine. 2007 Aug;14(7-8):563-7
pubmed: 17601714
Pharmacol Rev. 2003 Jun;55(2):325-95
pubmed: 12773631
Nat Rev Gastroenterol Hepatol. 2016 Mar;13(3):131-49
pubmed: 26837712
J Zhejiang Univ Sci B. 2016 Dec.;17(12):941-951
pubmed: 27921399
J Gastroenterol Hepatol. 2011 Jul;26(7):1089-105
pubmed: 21545524
J Neurosci. 2011 Oct 12;31(41):14496-507
pubmed: 21994366
Evid Based Complement Alternat Med. 2017;2017:6125829
pubmed: 28280515
Br J Pharmacol. 2014 Oct;171(20):4575-94
pubmed: 24611635
J Pharm Pharmacol. 2000 Apr;52(4):437-40
pubmed: 10813555
Oxid Med Cell Longev. 2014;2014:310504
pubmed: 25013541
Food Funct. 2013 Apr 30;4(5):794-802
pubmed: 23584161
PLoS One. 2013;8(2):e55860
pubmed: 23409069
Alcohol Clin Exp Res. 2018 Oct;42(10):1939-1950
pubmed: 30080258
Curr Pharm Des. 2016;22(31):4763-4779
pubmed: 27356774
Biochem Biophys Res Commun. 2010 Nov 5;402(1):116-22
pubmed: 20932821
Stroke. 2009 May;40(5):1877-85
pubmed: 19299628
Am J Med. 2017 Feb;130(2):124-134
pubmed: 27984008
Int J Mol Sci. 2016 Sep 26;17(10):
pubmed: 27681723
Toxicol In Vitro. 2012 Aug;26(5):700-8
pubmed: 22484158
J Neurosci. 2014 Feb 19;34(8):2967-78
pubmed: 24553937
Pharmacol Biochem Behav. 2013 Sep;110:238-44
pubmed: 23932920
Int J Mol Sci. 2011;12(8):4770-80
pubmed: 21954324
Nature. 2000 Oct 12;407(6805):770-6
pubmed: 11048727
Food Funct. 2017 Nov 15;8(11):4217-4228
pubmed: 29043342
Redox Biol. 2014;3:109-23
pubmed: 25465468
J Neurosci. 2008 Sep 17;28(38):9473-85
pubmed: 18799679
Biomed Pharmacother. 2018 Nov;107:721-728
pubmed: 30138894
Environ Toxicol Pharmacol. 2009 Jan;27(1):120-6
pubmed: 21783929
Free Radic Res. 2005 Mar;39(3):255-68
pubmed: 15788230
FASEB J. 2008 Mar;22(3):659-61
pubmed: 17942826
Int J Mol Sci. 2013 Aug 29;14(9):17680-93
pubmed: 23994834
Am J Gastroenterol. 2018 Feb;113(2):175-194
pubmed: 29336434
Food Funct. 2015 Oct;6(10):3359-72
pubmed: 26267818
World J Gastrointest Pharmacol Ther. 2015 Aug 6;6(3):59-72
pubmed: 26261734
Am J Transl Res. 2015 Jul 15;7(7):1189-202
pubmed: 26328004
Adv Exp Med Biol. 1980;126:365-84
pubmed: 6996453
Food Funct. 2015 Dec;6(12):3760-9
pubmed: 26412138
Exp Mol Pathol. 2011 Jun;90(3):295-9
pubmed: 21352821
Int J Biol Sci. 2015 Apr 03;11(5):569-86
pubmed: 25892964
Drug Chem Toxicol. 2018 Sep 21;:1-7
pubmed: 30239227
Nutrition. 2006 Feb;22(2):166-73
pubmed: 16459229
Zhongguo Zhong Yao Za Zhi. 2003 Aug;28(8):756-8, 793
pubmed: 15015361
Transplant Proc. 2012 May;44(4):974-7
pubmed: 22564600
Liver Int. 2009 Nov;29(10):1457-66
pubmed: 19811613
Oxid Med Cell Longev. 2019 May 12;2019:9056845
pubmed: 31214283
Free Radic Biol Med. 2009 Jan 15;46(2):271-81
pubmed: 19015023
Dig Liver Dis. 2007 Apr;39(4):293-304
pubmed: 17331820
Toxicol Appl Pharmacol. 2008 May 15;229(1):1-9
pubmed: 18396304
Nutrients. 2018 Jul 01;10(7):
pubmed: 29966389
Environ Sci Pollut Res Int. 2016 Dec;23(24):25191-25199
pubmed: 27680006
J Sci Food Agric. 2015 Mar 15;95(4):799-808
pubmed: 25200286
Sci Rep. 2016 Oct 12;6:35055
pubmed: 27731352
Int J Mol Sci. 2017 Mar 23;18(4):
pubmed: 28333073
Wei Sheng Yan Jiu. 2014 Mar;43(2):282-5
pubmed: 24868984
Int Immunopharmacol. 2015 Apr;25(2):332-9
pubmed: 25681284
Cell Cycle. 2015;14(11):1631-42
pubmed: 25927598