Development of a novel murine model of lymphatic metastasis.
Animals
Cell Line, Tumor
/ transplantation
Colonic Neoplasms
/ pathology
Disease Models, Animal
Female
Green Fluorescent Proteins
/ chemistry
Humans
Luciferases
/ chemistry
Luminescent Measurements
Lymph Nodes
/ diagnostic imaging
Lymphatic Metastasis
/ diagnostic imaging
Lymphatic Vessels
Male
Mesentery
Mice
Mice, Inbred C57BL
Stomach Neoplasms
/ pathology
Tomography, Optical
Tumor Burden
Colon cancer
Gastric cancer
Intralymphatic
Luciferase
Mesenteric lymph duct
Journal
Clinical & experimental metastasis
ISSN: 1573-7276
Titre abrégé: Clin Exp Metastasis
Pays: Netherlands
ID NLM: 8409970
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
09
08
2019
accepted:
27
01
2020
pubmed:
14
2
2020
medline:
17
9
2020
entrez:
14
2
2020
Statut:
ppublish
Résumé
Current laboratory models of lymphatic metastasis generally require either genetically modified animals or are technically challenging. Herein, we have developed a robust protocol for the induction of intralymphatic metastasis in wild-type mice with reproducible outcomes. To determine an optimal injection quantity and timeline for tumorigenesis, C57Bl/6 mice were injected directly into the mesenteric lymph duct (MLD) with varying numbers of syngeneic murine colon cancer cells (MC38) or gastric cancer cells (YTN16) expressing GFP/luciferase and monitored over 2-4 weeks. Tumor growth was tracked via whole-animal in vivo bioluminescence imaging (IVIS). Our data indicate that the injection of tumor cells into the MLD is a viable model for lymphatic metastasis as necropsies revealed large tumor burdens and metastasis in regional lymph nodes. This protocol enables a closer study of the role of lymphatics in cancer metastasis and opens a window for the development of novel approaches for treatment of metastatic diseases.
Identifiants
pubmed: 32052231
doi: 10.1007/s10585-020-10025-3
pii: 10.1007/s10585-020-10025-3
pmc: PMC7979265
mid: NIHMS1680045
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
Luciferases
EC 1.13.12.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
247-255Subventions
Organisme : NIH HHS
ID : S10 OD021804
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA106183
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK058404
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA068485
Pays : United States
Organisme : NIDDK NIH HHS
ID : U25 DK059632
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK059637
Pays : United States
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