The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability.
GTP cap
biochemistry
cell biology
chemical biology
cytoskeleton
dynamic instability
end binding proteins
human
microtubule
tubulin
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
13 02 2020
13 02 2020
Historique:
received:
18
09
2019
accepted:
25
01
2020
entrez:
14
2
2020
pubmed:
14
2
2020
medline:
24
4
2021
Statut:
epublish
Résumé
Microtubules are cytoskeletal polymers whose function depends on their property to switch between states of growth and shrinkage. Growing microtubules are thought to be stabilized by a GTP cap at their ends. The nature of this cap, however, is still poorly understood. End Binding proteins (EBs) recruit a diverse range of regulators of microtubule function to growing microtubule ends. Whether the EB binding region is identical to the GTP cap is unclear. Using mutated human tubulin with blocked GTP hydrolysis, we demonstrate that EBs bind with high affinity to the GTP conformation of microtubules. Slowing-down GTP hydrolysis leads to extended GTP caps. We find that cap length determines microtubule stability and that the microtubule conformation changes gradually in the cap as GTP is hydrolyzed. These results demonstrate the critical importance of the kinetics of GTP hydrolysis for microtubule stability and establish that the GTP cap coincides with the EB-binding region.
Identifiants
pubmed: 32053491
doi: 10.7554/eLife.51992
pii: 51992
pmc: PMC7018511
doi:
pii:
Substances chimiques
Tubulin
0
Guanosine Triphosphate
86-01-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Cancer Research UK
ID : FC001163
Pays : United Kingdom
Organisme : Medical Research Council
ID : FC001163
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001163
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 100145/Z/12/Z
Pays : United Kingdom
Organisme : European Research Council
ID : 323042
Pays : International
Informations de copyright
© 2020, Roostalu et al.
Déclaration de conflit d'intérêts
JR, CT, NC, SK, IT No competing interests declared, TS Reviewing Editor, eLife
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