Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence.
Abortion, Spontaneous
/ epidemiology
Adolescent
Adult
Animals
Bacteria
/ classification
Female
Gram-Negative Bacterial Infections
/ complications
Humans
Mycoplasma Infections
/ complications
Mycoplasma hominis
/ isolation & purification
Seroepidemiologic Studies
Tunisia
/ epidemiology
Vagina
/ microbiology
Young Adult
Zoonoses
/ complications
Waddlia
genital infection
genital mycoplasmas
miscarriage
zoonosis
Journal
Journal of infection in developing countries
ISSN: 1972-2680
Titre abrégé: J Infect Dev Ctries
Pays: Italy
ID NLM: 101305410
Informations de publication
Date de publication:
31 05 2019
31 05 2019
Historique:
received:
13
10
2017
accepted:
18
10
2018
entrez:
14
2
2020
pubmed:
14
2
2020
medline:
29
7
2020
Statut:
epublish
Résumé
Miscarriage is one of the most common adverse pregnancy outcomes. The aim of this study was to investigate the relationship between miscarriage in humans and infections caused by zoonotic bacteria and genital pathogens. Cervicovaginal swabs and placenta samples from 132 women with miscarriage (patient group: PG), and cervicovaginal swabs from 54 women with normal pregnancy (control group:CG), were subjected to bacteriological culture and real time PCRs detecting Coxiella burnetii, Brucella spp, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Chlamydia trachomatis, Waddlia chondrophila and Parachlamydia acanthamoebeae DNA. Serology of C. burnetii, C. trachomatis and W. chondrophila was also performed. Placenta samples were positive for E. coli, S. agalactiae, U. urealyticum, M. hominis and C. trachomatis in 4.7%, 3.1%, 3.1%, 0.7% and 0.7% of cases, respectively. For cervicovaginal swabs, M. hominis was more frequently detected among PG than CG with a significant statistical difference (p = 0.02). C. trachomatis was detected in 3.3% and 5.5% among PG and CG, respectively. U. urealyticum DNA was detected with high percentages in the two groups. Samples from both groups showed negatives results for C. burnetii, Waddlia, and Brucella qPCRs. A high rate of W. chondrophila seroprevalence (42%) was noted with significant difference among women with early miscarriage. C. trachomatis, S. agalactiae and M. hominis may play a role in miscarriage. However, the full characterization of the vaginal flora using other technologies such as NGS-based metagenomics is needed to clarify their role in miscarriage. Finally, further investigations should be performed to explain high W. chondrophila seroprevalence.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
410-418Informations de copyright
Copyright (c) 2019 Smaoui mariem, Kebbi Carole, Sellami Hanen, Kammoun Salma, Choura Khaled, Maazoun Leila, Mestiri Houssem, Aeby Sebastien, Louati Doulira, Derbel Mohamed, Chaabene Kais, Hammami Adnene, Greub Gilbert, Znazen Abir.
Déclaration de conflit d'intérêts
No Conflict of Interest is declared