The crowding dynamics of the motor protein kinesin-II.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
14
08
2019
accepted:
26
01
2020
entrez:
14
2
2020
pubmed:
14
2
2020
medline:
4
6
2020
Statut:
epublish
Résumé
Intraflagellar transport (IFT) in C. elegans chemosensory cilia is an example of functional coordination and cooperation of two motor proteins with distinct motility properties operating together in large groups to transport cargoes: a fast and processive homodimeric kinesin-2, OSM-3, and a slow and less processive heterotrimeric kinesin-2, kinesin-II. To study the mechanism of the collective dynamics of kinesin-II of C. elegans cilia in an in vitro system, we used Total Internal Reflection Fluorescence microscopy to image the motility of truncated, heterodimeric kinesin-II constructs at high motor densities. Using an analysis technique based on correlation of the fluorescence intensities, we extracted quantitative motor parameters, such as motor density, velocity and average run length, from the image. Our experiments and analyses show that kinesin-II motility parameters are far less affected by (self) crowding than OSM-3. Our observations are supported by numerical calculations based on the TASEP-LK model (Totally Asymmetric Simple Exclusion Process-Langmuir Kinetics). From a comparison of data and modelling of OSM-3 and kinesin-II, a general picture emerges of the collective dynamics of the kinesin motors driving IFT in C. elegans chemosensory cilia and the way the motors deal with crowding.
Identifiants
pubmed: 32053680
doi: 10.1371/journal.pone.0228930
pii: PONE-D-19-22831
pmc: PMC7018031
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Molecular Motor Proteins
0
kinesin-II
147096-75-1
Myosins
EC 3.6.4.1
Dyneins
EC 3.6.4.2
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0228930Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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