3D mapping of native extracellular matrix reveals cellular responses to the microenvironment.
Collagen VI
Cryo electron microscopy
Extracellular matrix
Fibronectin
Scanning transmission electron microscopy
Tomography
Journal
Journal of structural biology: X
ISSN: 2590-1524
Titre abrégé: J Struct Biol X
Pays: United States
ID NLM: 101761384
Informations de publication
Date de publication:
Historique:
received:
16
07
2018
revised:
23
11
2018
accepted:
07
12
2018
entrez:
15
2
2020
pubmed:
15
2
2020
medline:
15
2
2020
Statut:
ppublish
Résumé
Cells and extracellular matrix (ECM) are mutually interdependent: cells guide self-assembly of ECM precursors, and the resulting ECM architecture supports and instructs cells. Though bidirectional signaling between ECM and cells is fundamental to cell biology, it is challenging to gain high-resolution structural information on cellular responses to the matrix microenvironment. Here we used cryo-scanning transmission electron tomography (CSTET) to reveal the nanometer- to micron-scale organization of major fibroblast ECM components in a native-like context, while simultaneously visualizing internal cell ultrastructure including organelles and cytoskeleton. In addition to extending current models for collagen VI fibril organization, three-dimensional views of thick cell regions and surrounding matrix showed how ECM networks impact the structures and dynamics of intracellular organelles and how cells remodel ECM. Collagen VI and fibronectin were seen to distribute in fundamentally different ways in the cell microenvironment and perform distinct roles in supporting and interacting with cells. This work demonstrates that CSTET provides a new perspective for the study of ECM in cell biology, highlighting labeled extracellular elements against a backdrop of unlabeled but morphologically identifiable cellular features with nanometer resolution detail.
Identifiants
pubmed: 32055794
doi: 10.1016/j.yjsbx.2018.100002
pii: S2590-1524(18)30002-3
pii: 100002
pmc: PMC7001979
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100002Informations de copyright
© 2018 The Authors.
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