NFKB2 gene expression in patients with peptic ulcer diseases and gastric cancer.
Expression
Gastric cancer
NFKB2
Peptic ulcer
Real-time PCR
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
19
11
2019
accepted:
31
01
2020
pubmed:
15
2
2020
medline:
21
10
2020
entrez:
15
2
2020
Statut:
ppublish
Résumé
Gastric cancer is one of the most common worldwide types of cancer. It is a multifactorial disease and both environmental and genetic factors play an important role in its etiology. Evaluation of the relative expression level of NFKB2 gene in two groups of patients: peptic ulcer and gastric cancer and its role in the pathomechanism of these diseases was the aim of this study. RNA was isolated from: 79 samples of peptic ulcer, 22 gastric cancer and 11 control tissue. The real-time PCR technique was used to study the expression of NFKB2 gene. The relative expression level of NFKB2 gene was a variable in all three studied groups. The relative NFKB2 gene expression depends on the type of a disease. Peptic ulcer cases showed the increased relative NFKB2 gene expression to control group (p = 0.0000). Cancer cases presented decreased relative NFKB2 gene expression to normal stomach tissue (p = 0.0183). There are statistically important differences in the investigated gene expression between peptic ulcer, where the expression level is higher comparing to gastric cancer and control tissue which confirmed that such an activation is connected with an inflammatory process. The relative expression level of NFKB2 is decreased in cancer cases as opposed to control tissue and peptic ulcer cases which could suggest that during carcinogenesis of gastric cancer inhibition of NF-kB pathway takes place which could be a promising factor for patients.
Identifiants
pubmed: 32056043
doi: 10.1007/s11033-020-05299-5
pii: 10.1007/s11033-020-05299-5
doi:
Substances chimiques
NF-kappa B p52 Subunit
0
NFKB2 protein, human
0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2015-2021Subventions
Organisme : Department of Pharmaceutical Biochemistry and Molecular Diagnostics
ID : 503/3-015-02/503-31-001
Organisme : Research task of the Medical University of Lodz
ID : 502-03/3-015-02/502-34-080
Organisme : Research task of the Medical University of Lodz
ID : 502-03/3-015-02/502-34-089
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