Transdermal colorimetric patch for hyperglycemia sensing in diabetic mice.

Animals Biosensing Techniques Colorimetry Diabetes Mellitus, Experimental Glucose Glucose Oxidase Hydrogen Peroxide Hyperglycemia Limit of Detection Mice
Colorimetric sensor Enzyme biomineralization Glucose sensor Glucose-responsive Microneedle

Journal

Biomaterials
ISSN: 1878-5905
Titre abrégé: Biomaterials
Pays: Netherlands
ID NLM: 8100316

Informations de publication

Date de publication:
04 2020
Historique:
received: 23 10 2019
revised: 27 12 2019
accepted: 12 01 2020
pubmed: 15 2 2020
medline: 15 5 2021
entrez: 15 2 2020
Statut: ppublish

Résumé

The integration of sampling and instant metabolite readout can fundamentally elevate patient compliance. To circumvent the need for complex in-lab apparatus, here, an all-in-one sampling and display transdermal colorimetric microneedle patch was developed for sensing hyperglycemia in mice. The coloration of 3,3',5,5'-tetramethylbenzidine (TMB) is triggered by the cascade enzymatic reactions of glucose oxidase (GOx) and horseradish peroxidase (HRP) at abnormally high glucose levels. The HRP in the upper layer is biomineralized with calcium phosphate (CaP) shell to add a pH responsive feature for increased sensitivity as well as protection from nonspecific reactions. This colorimetric sensor achieved minimally invasive extraction of the interstitial fluid from mice and converted glucose level to a visible color change promptly. Quantitative red green and blue (RGB) information could be obtained through a scanned image of the microneedle. This costless, portable colorimetric sensor could potentially detect daily glucose levels without blood drawing procedures.

Identifiants

DOI: 10.1016/j.biomaterials.2020.119782 PMID: 32058155
pubmed: 32058155
pii: S0142-9612(20)30028-4
doi: 10.1016/j.biomaterials.2020.119782
pii:
doi:

Substances chimiques

Hydrogen Peroxide BBX060AN9V
Glucose Oxidase EC 1.1.3.4
Glucose IY9XDZ35W2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

119782

Informations de copyright

Copyright © 2020. Published by Elsevier Ltd.

Auteurs

Zejun Wang (Z)

Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States.

Hongjun Li (H)

Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States.

Jinqiang Wang (J)

Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States.

Zhaowei Chen (Z)

Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States.

Guojun Chen (G)

Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States.

Di Wen (D)

Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States.

Amanda Chan (A)

Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States.

Zhen Gu (Z)

Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, 90095, United States; Center for Minimally Invasive Therapeutics, University of California, Los Angeles, CA, 90095, United States. Electronic address: guzhen@ucla.edu.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Transdermal colorimetric patch for...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Transdermal colorimetric patch for...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Titrimétrie › Colorimétrie › Transdermal colorimetric patch for...
questionsmedicales.fr › Techniques d'investigation › Modèles animaux › Diabète expérimental › Transdermal colorimetric patch for...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Transdermal colorimetric patch for...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Alcohol oxidoreductases › Glucose oxidase › Transdermal colorimetric patch for...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Transdermal colorimetric patch for...
questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Maladies métaboliques › Troubles du métabolisme du glucose › Hyperglycémie › Transdermal colorimetric patch for...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Limite de détection › Transdermal colorimetric patch for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Transdermal colorimetric patch for...