Identifying neural substrates of competitive interactions and sequence transitions during mechanosensory responses in Drosophila.
Action Potentials
/ physiology
Animals
Animals, Genetically Modified
Binding, Competitive
/ physiology
Brain
/ anatomy & histology
Brain Mapping
Cues
Drosophila melanogaster
/ genetics
Mechanotransduction, Cellular
/ physiology
Neural Pathways
/ metabolism
Neurons
/ metabolism
Phenotype
Sensory Receptor Cells
/ physiology
Synaptic Transmission
/ physiology
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
10
09
2019
accepted:
30
12
2019
revised:
21
04
2020
pubmed:
15
2
2020
medline:
12
6
2020
entrez:
15
2
2020
Statut:
epublish
Résumé
Nervous systems have the ability to select appropriate actions and action sequences in response to sensory cues. The circuit mechanisms by which nervous systems achieve choice, stability and transitions between behaviors are still incompletely understood. To identify neurons and brain areas involved in controlling these processes, we combined a large-scale neuronal inactivation screen with automated action detection in response to a mechanosensory cue in Drosophila larva. We analyzed behaviors from 2.9x105 larvae and identified 66 candidate lines for mechanosensory responses out of which 25 for competitive interactions between actions. We further characterize in detail the neurons in these lines and analyzed their connectivity using electron microscopy. We found the neurons in the mechanosensory network are located in different regions of the nervous system consistent with a distributed model of sensorimotor decision-making. These findings provide the basis for understanding how selection and transition between behaviors are controlled by the nervous system.
Identifiants
pubmed: 32059010
doi: 10.1371/journal.pgen.1008589
pii: PGENETICS-D-19-01524
pmc: PMC7173939
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008589Subventions
Organisme : Wellcome Trust
ID : 205050/A/16/Z
Pays : United Kingdom
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : Medical Research Council
ID : MC_UP_1201/20
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UP_1201/21
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 205050/Z/16/Z
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Cell Rep. 2012 Oct 25;2(4):991-1001
pubmed: 23063364
Nature. 2010 Nov 18;468(7322):394-9
pubmed: 20972420
Elife. 2016 May 13;5:
pubmed: 27177418
Curr Biol. 2019 Feb 4;29(3):426-434.e6
pubmed: 30661796
Hum Mov Sci. 2007 Aug;26(4):525-54
pubmed: 17698232
Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13612-7
pubmed: 23898199
Cell Rep. 2014 Aug 7;8(3):897-908
pubmed: 25088417
Annu Rev Neurosci. 2007;30:535-74
pubmed: 17600525
PLoS One. 2013 Aug 20;8(8):e71706
pubmed: 23977118
Behav Cogn Neurosci Rev. 2002 Jun;1(2):108-29
pubmed: 17715589
Nature. 2013 Jan 10;493(7431):221-5
pubmed: 23222543
Nat Methods. 2011 Jun 05;8(7):592-8
pubmed: 21642964
Curr Biol. 2012 Nov 20;22(22):2124-34
pubmed: 23103192
Elife. 2016 Feb 15;5:
pubmed: 26880545
Neuron. 2016 Aug 3;91(3):615-28
pubmed: 27427461
Rouxs Arch Dev Biol. 1987 Feb;196(2):69-77
pubmed: 28305460
Science. 2014 Apr 25;344(6182):386-92
pubmed: 24674869
Annu Rev Neurosci. 2010;33:269-98
pubmed: 20345247
Elife. 2015 Jun 16;4:
pubmed: 26077825
Elife. 2016 Mar 18;5:
pubmed: 26990779
Elife. 2018 Mar 12;7:
pubmed: 29528286
Nature. 2017 Aug 9;548(7666):175-182
pubmed: 28796202
Nat Commun. 2019 Jun 14;10(1):2654
pubmed: 31201326
Neuron. 1994 Jun;12(6):1195-206
pubmed: 8011334
Nature. 2004 Jul 29;430(6999):564-9
pubmed: 15282607
Elife. 2016 Nov 15;5:
pubmed: 27845623
Elife. 2017 Oct 23;6:
pubmed: 29058674
Elife. 2018 Aug 02;7:
pubmed: 30070205
Philos Trans R Soc Lond B Biol Sci. 2007 Sep 29;362(1485):1585-99
pubmed: 17428779
Cell. 2016 Oct 20;167(3):858-870.e19
pubmed: 27720450
Neuron. 2017 Dec 20;96(6):1373-1387.e6
pubmed: 29198754
J Neurosci. 2010 Aug 4;30(31):10465-71
pubmed: 20685989
Proc Natl Acad Sci U S A. 2015 Jun 2;112(22):E2967-76
pubmed: 25964354
Nat Neurosci. 2014 Jul;17(7):962-70
pubmed: 24908103
Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9715-20
pubmed: 18621688
Nature. 2015 Apr 30;520(7549):633-9
pubmed: 25896325
Elife. 2014 Aug 19;3:e02951
pubmed: 25139955
J Neurosci. 2012 Sep 5;32(36):12460-71
pubmed: 22956837
J Neurosci. 2006 Oct 18;26(42):10925-33
pubmed: 17050731
Nat Neurosci. 2009 Nov;12(11):1450-7
pubmed: 19801989
Neuron. 1995 Feb;14(2):341-51
pubmed: 7857643
Curr Opin Neurobiol. 2012 Dec;22(6):927-36
pubmed: 22683275
Development. 2007 Jan;134(1):55-64
pubmed: 17164414
Neuron. 2015 Oct 21;88(2):314-29
pubmed: 26439528
Psychol Res. 2009 Jul;73(4):559-77
pubmed: 19347359
Cereb Cortex. 2007 Jan;17(1):44-62
pubmed: 16452643
Bioinformatics. 2009 Aug 1;25(15):1984-6
pubmed: 19376822
Nat Methods. 2013 Jan;10(1):64-7
pubmed: 23202433
J Neurosci. 1995 Mar;15(3 Pt 1):1755-67
pubmed: 7891133
Curr Biol. 2019 Feb 18;29(4):554-566.e4
pubmed: 30744969
Curr Biol. 2008 Oct 14;18(19):R928-32
pubmed: 18957243