tpHusion: An efficient tool for clonal pH determination in Drosophila.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
08
11
2019
accepted:
27
01
2020
entrez:
15
2
2020
pubmed:
15
2
2020
medline:
6
5
2020
Statut:
epublish
Résumé
Genetically encoded pH indicators (GEpHI) have emerged as important tools for investigating intracellular pH (pHi) dynamics in Drosophila. However, most of the indicators are based on the Gal4/UAS binary expression system. Here, we report the generation of a ubiquitously-expressed GEpHI. The fusion protein of super ecliptic pHluorin and FusionRed was cloned under the tubulin promoter (tpHusion) to drive it independently of the Gal4/UAS system. The function of tpHusion was validated in various tissues from different developmental stages of Drosophila. Differences in pHi were also indicated correctly in fixed tissues. Finally, we describe the use of tpHusion for comparative analysis of pHi in manipulated clones and the surrounding cells in epithelial tissues. Our findings establish tpHusion as a robust tool for studying pHi in Drosophila.
Identifiants
pubmed: 32059043
doi: 10.1371/journal.pone.0228995
pii: PONE-D-19-31154
pmc: PMC7021318
doi:
Substances chimiques
Drosophila Proteins
0
GAL4 protein, Drosophila
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0228995Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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