Metformin Improves Quality of Post-Thaw Canine Semen.
AMPK
canine sperm
cryopreservation
metformin
mitochondria
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
12 Feb 2020
12 Feb 2020
Historique:
received:
20
01
2020
revised:
07
02
2020
accepted:
07
02
2020
entrez:
16
2
2020
pubmed:
16
2
2020
medline:
16
2
2020
Statut:
epublish
Résumé
Sperm cryopreservation is an assisted reproductive technique routinely used in canine species for genetic conservation. However, during cryopreservation, the DNA damages are still elevated, limiting the fertilization rate. The present study was conducted to evaluate whether supplementation of canine semen extender with a molecule limiting the metabolic activities can improve the quality of frozen-thawed canine spermatozoa. We used metformin, known to limit the mitochondrial respiratory and limit the oxidative stress. Before and during the freezing procedure, metformin (50µM and 500µM) has been added to the extender. After thawing, sperm exposed to metformin conserved the same viability without alteration in the membrane integrity or acrosome reaction. Interestingly, 50µM metformin improved the sperm motility in comparison to the control, subsequently increasing mitochondrial activity and NAD
Identifiants
pubmed: 32059492
pii: ani10020287
doi: 10.3390/ani10020287
pmc: PMC7070956
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Region Centre Val de Loire
ID : Oxyferti
Organisme : Faculty of Veterinary Medicine, Wroclaw University
ID : -
Organisme : INRAE
ID : -
Déclaration de conflit d'intérêts
The authors declare no conflict of interest
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