mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules.
Animals
Codon Usage
Cytoplasmic Granules
/ metabolism
Down-Regulation
Endoplasmic Reticulum
/ metabolism
Endoribonucleases
/ genetics
Exoribonucleases
/ genetics
Mammals
/ genetics
Mutation
RNA, Messenger
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Stress, Physiological
/ physiology
Saccharomyces cerevisiae
mRNA accumulation
stress granules
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
12 Feb 2020
12 Feb 2020
Historique:
received:
17
12
2019
revised:
04
02
2020
accepted:
04
02
2020
entrez:
16
2
2020
pubmed:
16
2
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Stress granules and P bodies are cytoplasmic structures assembled in response to various stress factors and represent sites of temporary storage or decay of mRNAs. Depending on the source of stress, the formation of these structures may be driven by distinct mechanisms, but several stresses have been shown to stabilize mRNAs via inhibition of deadenylation. A recent study identified yeast gene deletion mutants with constitutive stress granules and elevated P bodies; however, the mechanisms which trigger its formation remain poorly understood. Here, we investigate the possibility of accumulating mRNA with mammalian codon bias, which we termed the model RNA, in these mutants. We found that the model RNA accumulates in
Identifiants
pubmed: 32059599
pii: ijms21041234
doi: 10.3390/ijms21041234
pmc: PMC7072924
pii:
doi:
Substances chimiques
RNA, Messenger
0
Saccharomyces cerevisiae Proteins
0
DCP2 protein, S cerevisiae
EC 3.1.-
Endoribonucleases
EC 3.1.-
Exoribonucleases
EC 3.1.-
XRN1 protein, S cerevisiae
EC 3.1.11.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Program of Région Centre-Val de Loire, "ARD 2020 Biomédicament"
ID : Project A1
Organisme : La Ligue contre le Cancer comité du Loiret
ID : 2014-2015
Déclaration de conflit d'intérêts
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; nor in the decision to publish the results.
Références
Mol Cell Biol. 1993 Oct;13(10):6102-13
pubmed: 8413212
J Cell Biol. 2010 May 31;189(5):813-27
pubmed: 20513766
Cell. 2015 Mar 12;160(6):1111-24
pubmed: 25768907
J Cell Sci. 2014 Aug 1;127(Pt 15):3373-81
pubmed: 24906800
Biochim Biophys Acta. 2000 Apr 25;1491(1-3):37-48
pubmed: 10760568
Nucleic Acids Res. 1990 May 25;18(10):3091-2
pubmed: 2190191
RNA. 2006 Oct;12(10):1835-45
pubmed: 16940550
Nat Rev Drug Discov. 2018 Apr;17(4):261-279
pubmed: 29326426
PLoS Comput Biol. 2013;9(7):e1003161
pubmed: 23935476
Mol Biol Cell. 2007 Jun;18(6):2274-87
pubmed: 17429074
J Cell Biol. 2011 Aug 22;194(4):581-96
pubmed: 21859862
Yeast. 1997 Jul;13(9):837-48
pubmed: 9234672
RNA. 2009 Jun;15(6):1110-20
pubmed: 19369426
Yeast. 2004 Jun;21(8):661-70
pubmed: 15197731
Mol Cell. 2009 Dec 25;36(6):932-41
pubmed: 20064460
Methods Enzymol. 1991;194:319-29
pubmed: 2005796
Yeast. 1994 Dec;10(13):1793-808
pubmed: 7747518
Mol Cell. 2011 Sep 16;43(6):973-81
pubmed: 21925385
Mol Biol Cell. 2000 Mar;11(3):833-48
pubmed: 10712503
Cancer Res. 2000 Feb 15;60(4):1028-34
pubmed: 10706120
Nat Struct Mol Biol. 2008 Dec;15(12):1263-71
pubmed: 19011635
Genetics. 2012 Jul;191(3):671-702
pubmed: 22785621
Microbiology. 1997 Feb;143 ( Pt 2):303-11
pubmed: 9043107
Microbiology. 2013 Mar;159(Pt 3):565-579
pubmed: 23306673
Curr Genet. 2006 Apr;49(4):205-17
pubmed: 16397765
Nature. 2014 Oct 2;514(7520):117-21
pubmed: 25119046
J Cell Sci. 2011 Jan 15;124(Pt 2):228-39
pubmed: 21172806
Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12203-7
pubmed: 12215496
Yeast. 1998 Jul;14(10):953-61
pubmed: 9717241
Nat Protoc. 2012 Feb 02;7(2):408-19
pubmed: 22301778
Yeast. 2004 Aug;21(11):947-62
pubmed: 15334558
PLoS One. 2013;8(1):e49783
pubmed: 23300942
Genes Dev. 1995 Oct 1;9(19):2421-32
pubmed: 7557393
Annu Rev Biophys Biomol Struct. 2007;36:413-34
pubmed: 17477840
Genetics. 2013 Jan;193(1):109-23
pubmed: 23105015
J Cell Biol. 2008 Nov 3;183(3):441-55
pubmed: 18981231
FEMS Yeast Res. 2015 Mar;15(2):
pubmed: 25673754
Cold Spring Harb Perspect Biol. 2012 Sep 01;4(9):a012286
pubmed: 22763747
Science. 2010 Feb 26;327(5969):1142-5
pubmed: 20185727
Curr Protoc Mol Biol. 2003 Aug;Chapter 27:Unit 27.3
pubmed: 18265330
PLoS Biol. 2012;10(6):e1001342
pubmed: 22719226
J Cell Biol. 2007 Nov 5;179(3):467-83
pubmed: 17984322
RNA. 2005 Apr;11(4):371-82
pubmed: 15703442
J Cell Sci. 2009 Jun 15;122(Pt 12):2078-88
pubmed: 19470581
Cell. 2013 Jun 20;153(7):1461-74
pubmed: 23791177
Mol Cell. 2004 Jul 23;15(2):303-13
pubmed: 15260981
Mol Biol Cell. 2010 Aug 1;21(15):2624-38
pubmed: 20519435
Biotechniques. 1990 Apr;8(4):398-403
pubmed: 1692716
Yeast. 1991 Aug-Sep;7(6):609-15
pubmed: 1767589
RNA. 2016 Aug;22(8):1200-14
pubmed: 27251550
Nat Protoc. 2007;2(1):1-4
pubmed: 17401330
J Biol Chem. 2008 Jun 27;283(26):17908-18
pubmed: 18424442