Study on the discrete dielectrophoresis for particle-cell separation.
Cell-particle separation
Dielectrophoresis
Duty cycle
Microfluidic device
Red blood cell
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
29
08
2019
revised:
07
02
2020
accepted:
11
02
2020
pubmed:
16
2
2020
medline:
23
3
2021
entrez:
16
2
2020
Statut:
ppublish
Résumé
This paper presents the application of the discrete dielectrophoretic force to separate polystyrene particles from red blood cells. The separation process employs a simple microfluidic device that is composed of interdigitated electrodes and a microchannel. The discrete dielectrophoretic force is generated by adjusting the duty cycle of the applied voltage. The electrodes make a tilt angle with the microchannel to change the moving direction of the red blood cells. By adjusting the voltage magnitude and duty cycle, we investigate the deflection of red blood cells and the variation of cell velocity along electrode edge under positive dielectrophoresis. The experiments with polystyrene particles show that the enrichment of the particles is greater than 150 times. The maximum separation efficiency is 97% for particle-to-cell number ratio equal to 1:2000 in the sample having high cell concentration. Using the appropriate applied voltage magnitude and duty cycle, the discrete dielectrophoretic force can prevent the clogging of microchannel while successfully separating the particles from the cells with high enrichment and efficiency. The proposed principle can be readily applied to dielectrophoresis-based devices for biomedical sample preparation or diagnosis such as the separation of rare or infected cells from a blood sample.
Identifiants
pubmed: 32060955
doi: 10.1002/elps.201900473
doi:
Substances chimiques
Polystyrenes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
991-1001Subventions
Organisme : National Research Council of Thailand (NRCT)
Pays : International
Organisme : Thailand Science Research and Innovation (TSRI)
Pays : International
Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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