Protective effect of Diosmin against benzo(a)pyrene-induced lung injury in Swiss Albino Mice.
Animals
Anti-Inflammatory Agents
/ pharmacology
Antioxidants
/ metabolism
Benzo(a)pyrene
/ toxicity
Caspase 3
/ metabolism
Cyclooxygenase 2
/ metabolism
Diosmin
/ pharmacology
Environmental Pollutants
/ toxicity
Lung
/ drug effects
Lung Injury
/ metabolism
Male
Mice
NF-kappa B
/ metabolism
Oxidative Stress
/ drug effects
benzo(a)pyrene
diosmin
inflammation
lung injury
oxidative stress
pulmonary toxicity
Journal
Environmental toxicology
ISSN: 1522-7278
Titre abrégé: Environ Toxicol
Pays: United States
ID NLM: 100885357
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
21
11
2019
revised:
03
01
2020
accepted:
04
02
2020
pubmed:
16
2
2020
medline:
23
6
2020
entrez:
16
2
2020
Statut:
ppublish
Résumé
Diosmin, a naturally occurring flavonoid commonly present in citrus fruit, is known to exhibit anti-inflammatory, antimutagenic, antioxidant, and free radical scavenging as well as blood lipid lowering activities among others. Diosmin has also been used for the treatment of various diseases including diabetes mellitus and Alzheimer's disease. Our study explores the role of Diosmin in pulmonary toxicity (lung injury) induced by environmental contaminant benzo(a)pyrene [B(a)P]. Swiss Albino Mice (SAM) were administered with either Diosmin 100 or 200 mg/kg body weight daily for 14 days and then challenged with a single dose of B(a)P. On the 15th day, animals were sacrificed; lung tissues and blood were collected for molecular analysis. B(a)P administration in mice induced the thickening of lung epithelium, damaged alveolar architecture, and promoted inflammatory cell infiltration in the lung tissues. Also, B[a]P significantly increased the expression of NF-kB, COX-2, IL-6, Bax, cleaved caspase 3, and cleaved PARP proteins and decreased antioxidant enzyme levels. Diosmin-100 and Diosmin-200 significantly attenuated the damage to lung epithelium, alveolar architecture, and reduced inflammatory cell infiltration in the lung tissues of mice. Diosmin significantly (P < .05) attenuated the levels of oxidative stress markers: lactate dehydrogenase and xanthine oxidase. A decrease in expression of NF-kB, COX-2, IL-6, Bax, cleaved caspase 3, and cleaved PARP proteins in mice was challenged with B[a]P. Diosmin thus could be a promising therapeutic adjuvant against B[a]P-induced oxidative stress and lung damage.
Substances chimiques
Anti-Inflammatory Agents
0
Antioxidants
0
Environmental Pollutants
0
NF-kappa B
0
Benzo(a)pyrene
3417WMA06D
Diosmin
7QM776WJ5N
Cyclooxygenase 2
EC 1.14.99.1
Caspase 3
EC 3.4.22.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
747-757Informations de copyright
© 2020 Wiley Periodicals, Inc.
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